Core 2 will provide the five projects with a number of unique advanced optical systems;collaborate with the project investigators in the design, execution, and analysis of animal experiments;and develop new instrumentation and methodology as needs arise. The Core will be established in Boston within the Wellman Center for Photomedicine at Massachusetts General Hospital (MGH), where the new techniques and pilot experiments will be carried out. However, routine intravital microscopy experiments will be carried out in Houston within the new CABIR building where ad-hoc facilities have been specifically designed. The Advanced Intravital Microscopy Core is lead by Dr. Yun with the collaboration of Dr. Richards-Kortum at Rice University and Dr. Lin. Dr. Yun (Ph.D. Physics) is an Assistant Professor at Harvard Medical School within the Wellman Center for Photomedicine of the Massachusetts General Hospital. The activity of the core will be co-developed in collaboration with Dr. Lin within the Wellman Center for Photomedicine Dr. Yun and Dr. Lin research groups comprise a total of about 20 postdoctoral fellows and graduate students, externally funded, and in the past 3 years have published papers in Nature, Nature Medicine, Nature Photonics, Blood, Journal of Experimental Medicine, as well as top optics journals. Dr. Richards-Kortum (Ph.D. Physics) is Stanley C. Moore Professor and chair of the Bioengineering Department at Rice University. The Advanced Intravital Microscopy Core will use its technological tools and expertise to (i) reconstruct the vascular architecture (diameter, length, tortuosity, permeability) and the hydrodynamic conditions (flow rate, shear stress at the wall) within the region of interest;(ii) to visualize the transport of nano- and micro-sized particles within the vasculature and tumor microenvironment measuring in particulat their rate of adhesion to the endothelium, rate of extravasation from the vascular compartment, rate of diffusion within the epithelium, rate of internalization and possible exocytosis from normal and tumor cells. The transport of nanoparticles will be analyzed using the miniature front-view probe via minimally-invasive laparotomy.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54CA143837-06
Application #
8564201
Study Section
Special Emphasis Panel (ZCA1-SRLB-9 (O1))
Project Start
2009-09-28
Project End
2015-07-31
Budget Start
2013-09-19
Budget End
2014-07-31
Support Year
6
Fiscal Year
2013
Total Cost
$212,764
Indirect Cost
$52,380
Name
Methodist Hospital Research Institute
Department
Type
DUNS #
185641052
City
Houston
State
TX
Country
United States
Zip Code
77030
Pandolfi, Laura; Minardi, Silvia; Taraballi, Francesca et al. (2016) Composite microsphere-functionalized scaffold for the controlled release of small molecules in tissue engineering. J Tissue Eng 7:2041731415624668
Ware, Matthew J; Keshishian, Vazrik; Law, Justin J et al. (2016) Generation of an in vitro 3D PDAC stroma rich spheroid model. Biomaterials 108:129-42
Lapin, Norman A; Krzykawska-Serda, Martyna; Ware, Matthew J et al. (2016) Intravital microscopy for evaluating tumor perfusion of nanoparticles exposed to non-invasive radiofrequency electric fields. Cancer Nanotechnol 7:5
Tanei, Tomonori; Leonard, Fransisca; Liu, Xuewu et al. (2016) Redirecting Transport of Nanoparticle Albumin-Bound Paclitaxel to Macrophages Enhances Therapeutic Efficacy against Liver Metastases. Cancer Res 76:429-39
McConnell, Kellie I; Shamsudeen, Sabeel; Meraz, Ismail M et al. (2016) Reduced Cationic Nanoparticle Cytotoxicity Based on Serum Masking of Surface Potential. J Biomed Nanotechnol 12:154-64
Scott, Bronwyn; Shen, Jianliang; Nizzero, Sara et al. (2016) A pyruvate decarboxylase-mediated therapeutic strategy for mimicking yeast metabolism in cancer cells. Pharmacol Res 111:413-21
Mi, Yu; Mu, Chaofeng; Wolfram, Joy et al. (2016) A Micro/Nano Composite for Combination Treatment of Melanoma Lung Metastasis. Adv Healthc Mater 5:936-46
Corbo, Claudia; Molinaro, Roberto; Parodi, Alessandro et al. (2016) The impact of nanoparticle protein corona on cytotoxicity, immunotoxicity and target drug delivery. Nanomedicine (Lond) 11:81-100
Liu, Zongbin; Han, Xin; Qin, Lidong (2016) Recent Progress of Microfluidics in Translational Applications. Adv Healthc Mater 5:871-88
Leonard, Fransisca; Godin, Biana (2016) 3D In Vitro Model for Breast Cancer Research Using Magnetic Levitation and Bioprinting Method. Methods Mol Biol 1406:239-51

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