The patient tissue and blood sampling strategies are identical for all three Research Projects to allow the collection of variables from orthogonal measurements on the same samples. The Clinical Sample Core (CSC) will serve as the interface between the clinical cancer centers and the Research Proj ects. In particular, the CSC will be responsible for collecting, processing, and tracking blood samples and tissue samples and distributing them to the Research Projects in the appropriate format requested by Investigators. The CSC personnel are trained and will be directly supervised by the senior co-Lead investigator Dr. Kelly Bethel, who will also provide the communications interface to the medical oncology and research project investigators. The CSC is also responsible for all human subject regulations. For patient cohort 1, the CSC will deliver tissue samples from various sites of the primary tumor and regional metastases, as well as circulating tumor cells from blood samples in both patients with lung cancer and colon cancer. Fo r patient cohort 2, the CSC will provide CTCs isolated from the blood of 150 newly diagnosed patients with colon cancer and 150 newly diagnosed patients with lung cancer. For each of the tumor types, 50 of the patients will have eariy stage cancers at the time of diagnosis (l-ll); 50 patients will have midstage (III) cancers at the time of diagnosis, and 50 will be patients presenting with metastatic stage disease.

Public Health Relevance

The CSC is composed of medical oncology, pathology, and surgery experts across three cancer centers. This will guarantee a high quality ofthe clinical program and sample preparation for the greatest likelihood of success in the proposed three Research Proj ects. The team also has the expertise and experience to deli ver human samples to each Invesgator in the form factor necessary to make their measurements.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Specialized Center--Cooperative Agreements (U54)
Project #
Application #
Study Section
Special Emphasis Panel (ZCA1-SRLB-9 (O1))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Scripps Research Institute
La Jolla
United States
Zip Code
West, Jeffrey; Newton, Paul K (2017) Chemotherapeutic Dose Scheduling Based on Tumor Growth Rates Provides a Case for Low-Dose Metronomic High-Entropy Therapies. Cancer Res 77:6717-6728
Kuhn, P; Keating, S M; Baxter, G T et al. (2017) Lessons Learned: Transfer of the High-Definition Circulating Tumor Cell Assay Platform to Development as a Commercialized Clinical Assay Platform. Clin Pharmacol Ther 102:777-785
Carlsson, Anders; Kuhn, Peter; Luttgen, Madelyn S et al. (2017) Paired High-Content Analysis of Prostate Cancer Cells in Bone Marrow and Blood Characterizes Increased Androgen Receptor Expression in Tumor Cell Clusters. Clin Cancer Res 23:1722-1732
West, Jeffrey; Hasnain, Zaki; Mason, Jeremy et al. (2016) The prisoner's dilemma as a cancer model. Converg Sci Phys Oncol 2:
Mitrugno, Annachiara; Tormoen, Garth W; Kuhn, Peter et al. (2016) The prothrombotic activity of cancer cells in the circulation. Blood Rev 30:11-9
Baker-Groberg, Sandra M; Phillips, Kevin G; Healy, Laura D et al. (2015) Critical behavior of subcellular density organization during neutrophil activation and migration. Cell Mol Bioeng 8:543-552
King, Michael R; Phillips, Kevin G; Mitrugno, Annachiara et al. (2015) A physical sciences network characterization of circulating tumor cell aggregate transport. Am J Physiol Cell Physiol 308:C792-802
Phillips, Kevin G; Lee, Angela M; Tormoen, Garth W et al. (2015) The thrombotic potential of circulating tumor microemboli: computational modeling of circulating tumor cell-induced coagulation. Am J Physiol Cell Physiol 308:C229-36
Ruiz, Carmen; Li, Julia; Luttgen, Madelyn S et al. (2015) Limited genomic heterogeneity of circulating melanoma cells in advanced stage patients. Phys Biol 12:016008
Phillips, Kevin G; Baker-Groberg, Sandra M; McCarty, Owen J T (2014) Quantitative optical microscopy: measurement of cellular biophysical features with a standard optical microscope. J Vis Exp :

Showing the most recent 10 out of 60 publications