The patient tissue and blood sampling strategies are identical for all three Research Projects to allow the collection of variables from orthogonal measurements on the same samples. The Clinical Sample Core (CSC) will serve as the interface between the clinical cancer centers and the Research Proj ects. In particular, the CSC will be responsible for collecting, processing, and tracking blood samples and tissue samples and distributing them to the Research Projects in the appropriate format requested by Investigators. The CSC personnel are trained and will be directly supervised by the senior co-Lead investigator Dr. Kelly Bethel, who will also provide the communications interface to the medical oncology and research project investigators. The CSC is also responsible for all human subject regulations. For patient cohort 1, the CSC will deliver tissue samples from various sites of the primary tumor and regional metastases, as well as circulating tumor cells from blood samples in both patients with lung cancer and colon cancer. Fo r patient cohort 2, the CSC will provide CTCs isolated from the blood of 150 newly diagnosed patients with colon cancer and 150 newly diagnosed patients with lung cancer. For each of the tumor types, 50 of the patients will have eariy stage cancers at the time of diagnosis (l-ll); 50 patients will have midstage (III) cancers at the time of diagnosis, and 50 will be patients presenting with metastatic stage disease.

Public Health Relevance

The CSC is composed of medical oncology, pathology, and surgery experts across three cancer centers. This will guarantee a high quality ofthe clinical program and sample preparation for the greatest likelihood of success in the proposed three Research Proj ects. The team also has the expertise and experience to deli ver human samples to each Invesgator in the form factor necessary to make their measurements.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54CA143906-04
Application #
8555269
Study Section
Special Emphasis Panel (ZCA1-SRLB-9 (O1))
Project Start
2009-09-30
Project End
2014-08-31
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
4
Fiscal Year
2012
Total Cost
$705,376
Indirect Cost
$244,507
Name
Scripps Research Institute
Department
Type
DUNS #
781613492
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Mitrugno, Annachiara; Tormoen, Garth W; Kuhn, Peter et al. (2016) The prothrombotic activity of cancer cells in the circulation. Blood Rev 30:11-9
Ruiz, Carmen; Li, Julia; Luttgen, Madelyn S et al. (2015) Limited genomic heterogeneity of circulating melanoma cells in advanced stage patients. Phys Biol 12:016008
King, Michael R; Phillips, Kevin G; Mitrugno, Annachiara et al. (2015) A physical sciences network characterization of circulating tumor cell aggregate transport. Am J Physiol Cell Physiol 308:C792-802
Phillips, Kevin G; Lee, Angela M; Tormoen, Garth W et al. (2015) The thrombotic potential of circulating tumor microemboli: computational modeling of circulating tumor cell-induced coagulation. Am J Physiol Cell Physiol 308:C229-36
Baker-Groberg, Sandra M; Phillips, Kevin G; Healy, Laura D et al. (2015) Critical behavior of subcellular density organization during neutrophil activation and migration. Cell Mol Bioeng 8:543-552
Rodriguez-Lee, Mariam; Kuhn, Peter; Webb, David R (2014) Advancing cancer patient care by integrating circulating tumor cell technology to understand the spatial and temporal dynamics of cancer. Drug Dev Res 75:384-92
Totonchy, Jennifer E; Clepper, Lisa; Phillips, Kevin G et al. (2014) CXCR7 expression disrupts endothelial cell homeostasis and causes ligand-dependent invasion. Cell Adh Migr 8:165-76
Carlsson, Anders; Nair, Viswam S; Luttgen, Madelyn S et al. (2014) Circulating tumor microemboli diagnostics for patients with non-small-cell lung cancer. J Thorac Oncol 9:1111-9
Newton, Paul K; Mason, Jeremy; Hurt, Brian et al. (2014) Entropy, complexity, and Markov diagrams for random walk cancer models. Sci Rep 4:7558
Bethel, Kelly; Luttgen, Madelyn S; Damani, Samir et al. (2014) Fluid phase biopsy for detection and characterization of circulating endothelial cells in myocardial infarction. Phys Biol 11:016002

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