Project Overview: Research Project 2 is centered on the translational research of devising and applying novel and tunable magnetic nanoparticles (MNP), ultrasensitive magnetic sensor chips, and highly efficient magnetic sorters for cancer biomarkers and cells. As illustrated in Figure N3.2.1, the resulting magneto-nano protein chip platform will detect protein biomarkers down to attomolar concentrations (Aim 1.A) and will be used for monitoring therapeutic response of lung cancer (Aim 1.B) and early diagnosis of ovarian cancer (Aim 1.0). The resulting magnetic sifter (Aim 2.A) and tunable MNP (Aim 2.B) will be used to harvest circulating tumor cells from lung cancer patients or ovarian cancer stem cells with unprecedented capture and release efficiencies of >90% (Aim 2.C), critical for both cancer diagnosis and treatment. These rare cells will be interrogated with the nanotechnologies in Aim 1, as well as Project 3 (N3., by single cell protein and nucleic acid profiling and secretome analysis to further elucidate cancer pathways and metastasis (Aim 3). The application of these advances in nanotechnology to pre-clinical models and clinical subjects will lead to better understanding and treatment of cancer.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Specialized Center--Cooperative Agreements (U54)
Project #
Application #
Study Section
Special Emphasis Panel (ZCA1-GRB-S)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Stanford University
United States
Zip Code
Pu, Kanyi; Shuhendler, Adam J; Valta, Maija P et al. (2014) Phosphorylcholine-coated semiconducting polymer nanoparticles as rapid and efficient labeling agents for in vivo cell tracking. Adv Healthc Mater 3:1292-8
Hudak, Carolyn S; Gulyaeva, Olga; Wang, Yuhui et al. (2014) Pref-1 marks very early mesenchymal precursors required for adipose tissue development and expansion. Cell Rep 8:678-87
Cheng, Kai; Kothapalli, Sri-Rajasekhar; Liu, Hongguang et al. (2014) Construction and validation of nano gold tripods for molecular imaging of living subjects. J Am Chem Soc 136:3560-71
Zhang, Mingliang; Bechstein, Daniel J B; Wilson, Robert J et al. (2014) Wafer-scale synthesis of monodisperse synthetic magnetic multilayer nanorods. Nano Lett 14:333-8
Sinclair, Robert; Kempen, Paul Joseph; Chin, Richard et al. (2014) The Stanford Nanocharacterization Laboratory (SNL) and Recent Applications of an Aberration-Corrected Environmental Transmission Electron Microscope. Adv Eng Mater 16:476-481
Dimov, Ivan K; Lu, Rong; Lee, Eric P et al. (2014) Discriminating cellular heterogeneity using microwell-based RNA cytometry. Nat Commun 5:3451
Pu, Kanyi; Shuhendler, Adam J; Jokerst, Jesse V et al. (2014) Semiconducting polymer nanoparticles as photoacoustic molecular imaging probes in living mice. Nat Nanotechnol 9:233-9
Sasportas, Laura Sarah; Gambhir, Sanjiv Sam (2014) Imaging circulating tumor cells in freely moving awake small animals using a miniaturized intravital microscope. PLoS One 9:e86759
Park, Seung-Min; Sabour, Andrew F; Son, Jun Ho et al. (2014) Toward integrated molecular diagnostic system (i MDx): principles and applications. IEEE Trans Biomed Eng 61:1506-21
Wang, Jianbin; Quake, Stephen R (2014) RNA-guided endonuclease provides a therapeutic strategy to cure latent herpesviridae infection. Proc Natl Acad Sci U S A 111:13157-62

Showing the most recent 10 out of 57 publications