Project Overview: Research Project 2 is centered on the translational research of devising and applying novel and tunable magnetic nanoparticles (MNP), ultrasensitive magnetic sensor chips, and highly efficient magnetic sorters for cancer biomarkers and cells. As illustrated in Figure N3.2.1, the resulting magneto-nano protein chip platform will detect protein biomarkers down to attomolar concentrations (Aim 1.A) and will be used for monitoring therapeutic response of lung cancer (Aim 1.B) and early diagnosis of ovarian cancer (Aim 1.0). The resulting magnetic sifter (Aim 2.A) and tunable MNP (Aim 2.B) will be used to harvest circulating tumor cells from lung cancer patients or ovarian cancer stem cells with unprecedented capture and release efficiencies of >90% (Aim 2.C), critical for both cancer diagnosis and treatment. These rare cells will be interrogated with the nanotechnologies in Aim 1, as well as Project 3 (N3.3.5.2.1.3), by single cell protein and nucleic acid profiling and secretome analysis to further elucidate cancer pathways and metastasis (Aim 3). The application of these advances in nanotechnology to pre-clinical models and clinical subjects will lead to better understanding and treatment of cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54CA151459-05
Application #
8726313
Study Section
Special Emphasis Panel (ZCA1-GRB-S)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
5
Fiscal Year
2014
Total Cost
$236,780
Indirect Cost
Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
Park, Seung-Min; Lee, Jae Young; Hong, Soongweon et al. (2016) Dual transcript and protein quantification in a massive single cell array. Lab Chip 16:3682-8
Lee, Jung-Rok; Sato, Noriyuki; Bechstein, Daniel J B et al. (2016) Experimental and theoretical investigation of the precise transduction mechanism in giant magnetoresistive biosensors. Sci Rep 6:18692
SoRelle, Elliott D; Liba, Orly; Campbell, Jos L et al. (2016) A hyperspectral method to assay the microphysiological fates of nanomaterials in histological samples. Elife 5:
Sun, Ziyan; Cheng, Kai; Wu, Fengyu et al. (2016) Robust surface coating for a fast, facile fluorine-18 labeling of iron oxide nanoparticles for PET/MR dual-modality imaging. Nanoscale 8:19644-19653
Zhang, Ruiping; Cheng, Kai; Antaris, Alexander L et al. (2016) Hybrid anisotropic nanostructures for dual-modal cancer imaging and image-guided chemo-thermo therapies. Biomaterials 103:265-77
Koh, Ai Leen; Gidcumb, Emily; Zhou, Otto et al. (2016) Oxidation of Carbon Nanotubes in an Ionizing Environment. Nano Lett 16:856-63
Van de Sompel, Dominique; Sasportas, Laura S; Jokerst, Jesse V et al. (2016) Comparison of Deconvolution Filters for Photoacoustic Tomography. PLoS One 11:e0152597
Lee, Jung-Rok; Bechstein, Daniel J B; Ooi, Chin Chun et al. (2016) Magneto-nanosensor platform for probing low-affinity protein-protein interactions and identification of a low-affinity PD-L1/PD-L2 interaction. Nat Commun 7:12220
Lee, Jung-Rok; Haddon, D James; Wand, Hannah E et al. (2016) Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus. Sci Rep 6:27623
Liba, Orly; SoRelle, Elliott D; Sen, Debasish et al. (2016) Contrast-enhanced optical coherence tomography with picomolar sensitivity for functional in vivo imaging. Sci Rep 6:23337

Showing the most recent 10 out of 117 publications