A panel of 5 peptides will be developed that have specific binding activity to amplified and/or over expressed gene targets identified in Project 1. Validation of preferential binding will be performed to purified proteins and to cells in culture transfected with gene targets. Validation of selective binding will then be performed to high grade dysplasia and early adenocarcinoma in Barrett's esophagus on fresh endoscopic mucosal resection specimens and tissue biopsies in Core C using the multi-spectral scanning fiber endoscope (SFE) developed in Project 3. Rigorous statistical analysis will be performed by Core B. Phase 1 clinical studies of the individual peptides assigned to three fluorescent-labels will be performed to establish safety of this integrated imaging strategy for a future multi-center clinical validation study.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54CA163059-02
Application #
8555331
Study Section
Special Emphasis Panel (ZCA1-SRLB-1 (O1))
Project Start
2011-09-21
Project End
2016-08-31
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
2
Fiscal Year
2012
Total Cost
$213,101
Indirect Cost
$61,943
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Zhou, Juan; He, Lei; Pang, Zhijun et al. (2017) Identification and validation of FGFR2 peptide for detection of early Barrett's neoplasia. Oncotarget 8:87095-87106
Savastano, Luis E; Seibel, Eric J (2017) Scanning Fiber Angioscopy: A Multimodal Intravascular Imaging Platform for Carotid Atherosclerosis. Neurosurgery 64:188-198
Savastano, Luis E; Zhou, Quan; Smith, Arlene et al. (2017) Multimodal laser-based angioscopy for structural, chemical and biological imaging of atherosclerosis. Nat Biomed Eng 1:
Zhou, Quan; Li, Zhao; Zhou, Juan et al. (2016) In vivo photoacoustic tomography of EGFR overexpressed in hepatocellular carcinoma mouse xenograft. Photoacoustics 4:43-54
Joshi, Bishnu P; Wang, Thomas D (2016) Gastrointestinal imaging in 2015: Emerging trends in endoscopic imaging. Nat Rev Gastroenterol Hepatol 13:72-3
Ferrer-Torres, Daysha; Nancarrow, Derek J; Kuick, Rork et al. (2016) Genomic similarity between gastroesophageal junction and esophageal Barrett's adenocarcinomas. Oncotarget 7:54867-54882
Joshi, Bishnu P; Duan, Xiyu; Kwon, Richard S et al. (2016) Multimodal endoscope can quantify wide-field fluorescence detection of Barrett's neoplasia. Endoscopy 48:A1-A13
Joshi, Bishnu P; Zhou, Juan; Pant, Asha et al. (2016) Design and Synthesis of Near-Infrared Peptide for in Vivo Molecular Imaging of HER2. Bioconjug Chem 27:481-94
Sturm, Matthew B; Wang, Thomas D (2015) Emerging optical methods for surveillance of Barrett's oesophagus. Gut 64:1816-23
Jiang, Hui; Salzman, Julia (2015) A penalized likelihood approach for robust estimation of isoform expression. Stat Interface 8:437-445

Showing the most recent 10 out of 40 publications