A panel of 5 peptides will be developed that have specific binding activity to amplified and/or over expressed gene targets identified in Project 1. Validation of preferential binding will be performed to purified proteins and to cells in culture transfected with gene targets. Validation of selective binding will then be performed to high grade dysplasia and early adenocarcinoma in Barrett's esophagus on fresh endoscopic mucosal resection specimens and tissue biopsies in Core C using the multi-spectral scanning fiber endoscope (SFE) developed in Project 3. Rigorous statistical analysis will be performed by Core B. Phase 1 clinical studies of the individual peptides assigned to three fluorescent-labels will be performed to establish safety of this integrated imaging strategy for a future multi-center clinical validation study.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54CA163059-04
Application #
8724433
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
4
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
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Joshi, Bishnu P; Wang, Thomas D (2016) Gastrointestinal imaging in 2015: Emerging trends in endoscopic imaging. Nat Rev Gastroenterol Hepatol 13:72-3
Joshi, Bishnu P; Zhou, Juan; Pant, Asha et al. (2016) Design and Synthesis of Near-Infrared Peptide for in Vivo Molecular Imaging of HER2. Bioconjug Chem 27:481-94
Zhou, Quan; Li, Zhao; Zhou, Juan et al. (2016) In vivo photoacoustic tomography of EGFR overexpressed in hepatocellular carcinoma mouse xenograft. Photoacoustics 4:43-54
Zhou, Juan; Joshi, Bishnu P; Duan, Xiyu et al. (2015) EGFR Overexpressed in Colonic Neoplasia Can be Detected on Wide-Field Endoscopic Imaging. Clin Transl Gastroenterol 6:e101
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Jiang, Hui; Salzman, Julia (2015) A penalized likelihood approach for robust estimation of isoform expression. Stat Interface 8:437-445

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