The Validation &Pathology Core consists of personnel with expertise in clinical gastroenterology, advanced procedures (endoscopic mucosal resection, endoscopic ultrasound), specimen processing, molecular biology, and pathology. Core personnel have many years experience in validation methods. The specific role of this BETRNet Specialized Research Rescource (Core) is to support the Research Center in the design and analysis of validation studies for the binding activity of fluorescent-labeled peptides to high-grade dysplasia and early adenocarcinoma for imaging in Barrett's esophagus. This Core will also support the three Primary Research Projects, developmental (Pilot) projects, cross-BETRNet projects, and other cross-BETRNet activities by providing methods and services for pathological evaluation of tissue specimens, including EMR and biopsy. The Validation &Pathology Core is organized into two parts: 1) the validation group which consists of clinical investigators and support staff that will recruit human subjects, collect specimens of Barrett's esophagus, administer selected fluorescent-labeled peptides, collect fluorescence images, and register fluorescence intensities with histopathology. These studies will be performed to validate affinity binding of the panel of peptides selected against amplified and overexpressed genes identified on the genomic database. Validation is critical to selection of an optimized set of peptides to be validated in the Phase 1 clinical studies with the proposed novel targeted imaging strategy;and 2) the pathology group which consists of gastrointestinal pathologists who will provide rigorous histopathology evaluation of the esophageal specimens that will be registered with the fluorescence images to sub-millimeter resolution.

Agency
National Institute of Health (NIH)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54CA163059-04
Application #
8724437
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
City
Ann Arbor
State
MI
Country
United States
Zip Code
Joshi, Bishnu P; Duan, Xiyu; Kwon, Richard S et al. (2016) Multimodal endoscope can quantify wide-field fluorescence detection of Barrett's neoplasia. Endoscopy 48:A1-A13
Joshi, Bishnu P; Wang, Thomas D (2016) Gastrointestinal imaging in 2015: Emerging trends in endoscopic imaging. Nat Rev Gastroenterol Hepatol 13:72-3
Joshi, Bishnu P; Zhou, Juan; Pant, Asha et al. (2016) Design and Synthesis of Near-Infrared Peptide for in Vivo Molecular Imaging of HER2. Bioconjug Chem 27:481-94
Zhou, Quan; Li, Zhao; Zhou, Juan et al. (2016) In vivo photoacoustic tomography of EGFR overexpressed in hepatocellular carcinoma mouse xenograft. Photoacoustics 4:43-54
Zhou, Juan; Joshi, Bishnu P; Duan, Xiyu et al. (2015) EGFR Overexpressed in Colonic Neoplasia Can be Detected on Wide-Field Endoscopic Imaging. Clin Transl Gastroenterol 6:e101
Myers, Amy L; Lin, Lin; Nancarrow, Derek J et al. (2015) IGFBP2 modulates the chemoresistant phenotype in esophageal adenocarcinoma. Oncotarget 6:25897-916
Stachler, Matthew D; Taylor-Weiner, Amaro; Peng, Shouyong et al. (2015) Paired exome analysis of Barrett's esophagus and adenocarcinoma. Nat Genet 47:1047-55
Sturm, Matthew B; Wang, Thomas D (2015) Emerging optical methods for surveillance of Barrett's oesophagus. Gut 64:1816-23
Lin, Jules; Myers, Amy L; Wang, Zhuwen et al. (2015) Osteopontin (OPN/SPP1) isoforms collectively enhance tumor cell invasion and dissemination in esophageal adenocarcinoma. Oncotarget 6:22239-57
Jiang, Hui; Salzman, Julia (2015) A penalized likelihood approach for robust estimation of isoform expression. Stat Interface 8:437-445

Showing the most recent 10 out of 36 publications