The primary goal of the Quantitative Core is, firstly, to provide specialized equipment for the measurement of the mechanical properties of cells and the cellular microenvironment. Secondly, the Core aims to offer centralized expertise in the experimental approaches to studying the cellular responses to mechanical inputs, ranging from the molecular level at the cell extracellular matrix interface to cell- and tissue-level biochemical responses in 2-D and 3-D synthetic and natural organotypic cultures. The decision to organize this Core around the UCSF Department of Surgery Center for Bioengineering and Tissue Regeneration is a recognition of the wealth of expertise and specialized instrumentation in this area of study available within the Center and the need to make this more readily accessible to investigators with intersecting research interests. The organizational structure and interaction of the Quantitative Core with the rest of the UCSF TMEN Brain Cancer Center and other core facilities at UCSF is shown in the adjacent flow diagram. An Executive Committee consisting of a select number of the principal participants in the UCSF TMEN Brain Cancer Center will work closely with the Core Director, Valerie Weaver, PhD, to set priorities and requests for pilot projects. The daily operation of the Core will be overseen by the Core Manager, Johnathon Lakins, PhD. Dr. Lakins will also serve as the primary interface with other centralized cores at UCSF and in the Bay Area for projects deemed to require additional or complementary equipment and expertise. The Quantitative Core will build upon the already extensive collaborations that the Center for Bioengineering and Tissue Regeneration has developed with the Nano Fabrication Core, Biological Imaging Development Center (BIDC), and Nikon Imaging Center (NIC) at UCSF, and the Integrative Cancer Biology Program (ICBP) at Lawrence Berkeley National Laboratory (LBNL). Matthew Krummel, PhD, Associate Professor of Pathology and Director ofthe BIDC, will serve as an as-needs principal consultant on projects with a significant component of light microscopic imaging and image processing.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54CA163155-03
Application #
8741088
Study Section
Special Emphasis Panel (ZCA1-SRLB-3 (O1))
Project Start
2011-09-26
Project End
2016-07-31
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
3
Fiscal Year
2013
Total Cost
$13,660
Indirect Cost
$4,832
Name
University of California San Francisco
Department
Type
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Przybyla, L; Lakins, J N; Sunyer, R et al. (2016) Monitoring developmental force distributions in reconstituted embryonic epithelia. Methods 94:101-13
Yu, Dan; Dong, Zhiqiang; Gustafson, William Clay et al. (2016) Rational design of a monomeric and photostable far-red fluorescent protein for fluorescence imaging in vivo. Protein Sci 25:308-15
Kai, FuiBoon; Laklai, Hanane; Weaver, Valerie M (2016) Force Matters: Biomechanical Regulation of Cell Invasion and Migration in Disease. Trends Cell Biol 26:486-97
Ou, Guanqing; Thakar, Dhruv; Tung, Jason C et al. (2016) Visualizing mechanical modulation of nanoscale organization of cell-matrix adhesions. Integr Biol (Camb) 8:795-804
Swartling, Fredrik J; Čančer, Matko; Frantz, Aaron et al. (2015) Deregulated proliferation and differentiation in brain tumors. Cell Tissue Res 359:225-54
Tung, Jason C; Barnes, J Matthew; Desai, Shraddha R et al. (2015) Tumor mechanics and metabolic dysfunction. Free Radic Biol Med 79:269-80
Cassereau, Luke; Miroshnikova, Yekaterina A; Ou, Guanqing et al. (2015) A 3D tension bioreactor platform to study the interplay between ECM stiffness and tumor phenotype. J Biotechnol 193:66-9
Rivera, Lee B; Bergers, Gabriele (2015) Intertwined regulation of angiogenesis and immunity by myeloid cells. Trends Immunol 36:240-9
Rivera, Lee B; Meyronet, David; Hervieu, Valérie et al. (2015) Intratumoral myeloid cells regulate responsiveness and resistance to antiangiogenic therapy. Cell Rep 11:577-91
Hambardzumyan, Dolores; Bergers, Gabriele (2015) Glioblastoma: Defining Tumor Niches. Trends Cancer 1:252-265

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