Eli Lilly San Diego Center for Biotechnology in a purpose-built 12,000 square foot laboratory, equipped with all of the necessary instrumentation/computers, including 10 Temperature Controlled Shaker Incubators (capable of fermenting 180 liters per day), four 4-channel AKTAxpress Chromatography Robots plus 13 single channel AKTA Chromatography Systems, Applied Biosystems MALDI-TOF (Voyager DE-RP and Voyager DE-STR) Mass Spectrometers, Applied Biosystems ESI-Quadrupole Mass Spectrometer, ThermoFinnigan LCQ Deca XP Plus ESI-lontrap Mass Spectrometer for MS/MS analysis. Applied Biosystems Qtrap 4000 Mass Spectrometer, five Agilent 1100 HPLCs, Wyatt Minidawn Treso Multiangle Light Scattering and Optlab Refractive Index Detector System, an Aviv Model 215 CD Spectrometer, a Biacore T100, a Robbins Phoenix Crystallization Robot, and a Formulatrix Formulator robot for preparing optimization trays. The remaining 50% of protein crystallization will be conducted at AECOM in a purpose built, similarly equipped crystallization laboratory. X-ray diffraction screening and data collection will be conducted at the APS using Lilly's State-of-the-Art LRL-CAT insertion device beamline, which is equipped with a mosaic CCD area detector and cryogenic crystal handling/mounting robotics. During APS maintenance shutdowns (-25% of the calendar year), access to Beamline X29 at the National Synchrotron Light Source will permit uninterrupted access to X-ray data collection facilities and occasional opportunities for expert intervention for challenging diffraction data collection (as needed).

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Specialized Center--Cooperative Agreements (U54)
Project #
Application #
Study Section
Special Emphasis Panel (ZGM1-PPBC-3)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Illinois Urbana-Champaign
United States
Zip Code
Mashiyama, Susan T; Malabanan, M Merced; Akiva, Eyal et al. (2014) Large-scale determination of sequence, structure, and function relationships in cytosolic glutathione transferases across the biosphere. PLoS Biol 12:e1001843
Akiva, Eyal; Brown, Shoshana; Almonacid, Daniel E et al. (2014) The Structure-Function Linkage Database. Nucleic Acids Res 42:D521-30
Zheng, Heping; Hou, Jing; Zimmerman, Matthew D et al. (2014) The future of crystallography in drug discovery. Expert Opin Drug Discov 9:125-37
Wichelecki, Daniel J; Graff, Dylan C; Al-Obaidi, Nawar et al. (2014) Identification of the in vivo function of the high-efficiency D-mannonate dehydratase in Caulobacter crescentus NA1000 from the enolase superfamily. Biochemistry 53:4087-9
Dong, Guang Qiang; Calhoun, Sara; Fan, Hao et al. (2014) Prediction of substrates for glutathione transferases by covalent docking. J Chem Inf Model 54:1687-99
Wichelecki, Daniel J; Vendiola, Jean Alyxa Ferolin; Jones, Amy M et al. (2014) Investigating the physiological roles of low-efficiency D-mannonate and D-gluconate dehydratases in the enolase superfamily: pathways for the catabolism of L-gulonate and L-idonate. Biochemistry 53:5692-9
Bouvier, Jason T; Groninger-Poe, Fiona P; Vetting, Matthew et al. (2014) Galactaro ?-lactone isomerase: lactone isomerization by a member of the amidohydrolase superfamily. Biochemistry 53:614-6
Wichelecki, Daniel J; Froese, D Sean; Kopec, Jolanta et al. (2014) Enzymatic and structural characterization of rTS? provides insights into the function of rTS?. Biochemistry 53:2732-8
Pandya, Chetanya; Dunaway-Mariano, Debra; Xia, Yu et al. (2014) Structure-guided approach for detecting large domain inserts in protein sequences as illustrated using the haloacid dehalogenase superfamily. Proteins 82:1896-906
Kumar, Ritesh; Zhao, Suwen; Vetting, Matthew W et al. (2014) Prediction and biochemical demonstration of a catabolic pathway for the osmoprotectant proline betaine. MBio 5:e00933-13

Showing the most recent 10 out of 49 publications