2.1. PROJECT 2 (Wagner) ? New Labeling Schemes, NMR Technology, and MP Structure and Function The major objectives of this project are to develop MP reconstitution and isotope labeling methods and new NMR experiments for the proposed pipeline to facilitate MP structure determination by solution NMR. These new developments, in combination with methods to be established in other projects of the Center, will enable routine NMR structure determination of MPs and characterization of MP complexes.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54GM094608-05
Application #
8688795
Study Section
Special Emphasis Panel (ZGM1-CBB-3)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
5
Fiscal Year
2014
Total Cost
$304,314
Indirect Cost
$86,408
Name
Harvard Medical School
Department
Type
DUNS #
047006379
City
Boston
State
MA
Country
United States
Zip Code
02115
Schlebach, Jonathan P; Barrett, Paul J; Day, Charles A et al. (2016) Topologically Diverse Human Membrane Proteins Partition to Liquid-Disordered Domains in Phase-Separated Lipid Vesicles. Biochemistry 55:985-8
Oxenoid, Kirill; Dong, Ying; Cao, Chan et al. (2016) Architecture of the mitochondrial calcium uniporter. Nature 533:269-73
Kim, Ji-Hun; Schlebach, Jonathan P; Lu, Zhenwei et al. (2016) A pH-Mediated Topological Switch within the N-Terminal Domain of Human Caveolin-3. Biophys J 110:2475-85
Chou, James J; Wang, Jia-huai (2015) Transmembrane signaling: A multiplex problem with converging solutions. Prog Biophys Mol Biol 118:87-8
Brüschweiler, Sven; Yang, Qin; Run, Changqing et al. (2015) Substrate-modulated ADP/ATP-transporter dynamics revealed by NMR relaxation dispersion. Nat Struct Mol Biol 22:636-41
Chatterjee, Deep; Eckert, Carl Elias; Slavov, Chavdar et al. (2015) Influence of Arrestin on the Photodecay of Bovine Rhodopsin. Angew Chem Int Ed Engl 54:13555-60
Schlebach, Jonathan P; Sanders, Charles R (2015) Influence of Pathogenic Mutations on the Energetics of Translocon-Mediated Bilayer Integration of Transmembrane Helices. J Membr Biol 248:371-81
Deatherage, Catherine L; Lu, Zhenwei; Kim, Ji-Hun et al. (2015) Notch Transmembrane Domain: Secondary Structure and Topology. Biochemistry 54:3565-8
Dev, Jyoti; Brüschweiler, Sven; Ouyang, Bo et al. (2015) Transverse relaxation dispersion of the p7 membrane channel from hepatitis C virus reveals conformational breathing. J Biomol NMR 61:369-78
Milbradt, Alexander G; Arthanari, Haribabu; Takeuchi, Koh et al. (2015) Increased resolution of aromatic cross peaks using alternate 13C labeling and TROSY. J Biomol NMR 62:291-301

Showing the most recent 10 out of 61 publications