The Animal Core will serve as a resource to supply Center investigators with the necessary animals and animal research techniques to effectively execute their research. The Animal Core will be responsible for the purchasing and inventory of the animals used by Center investigators, as well as, providing investigators with the technical ability required in the manipulation of the various animal models. In the past the Animal Core was responsible for the generation of genetically engineered mice. However, the focus of the research in this center application will be on investigation of endometrial biology in mouse models already generated. Therefore, the emphasis of this Core in the renewal will be to serve as a repository for the animals used in the core and for the phenotypic analysis of impact of the genetic alterations on mouse reproductive biology. This Core will incorporate the use of xenotransplants of human endometrial tissue into NOD/SCID/yc nu"""""""" (NOG) mice, as well as, the use of mouse models for endometriosis. The Core will be responsible for the execution of these complicated techniques for the analysis of endometrial biology and will only generate new models as deemed necessary. The Core will serve Center Investigators by accomplishing the following tasks: 1. The Animal Core will provide the phenotypic analysis of the impact of specific genetic alterations on mouse reproductive biology. 2. The Animal Core will conduct xenograft procedures involved in the reconstitution of endometrial tissue into mice. 3. The Animal Core will manage the colonies of genetically engineered mice for Center Investigators. 4. The Animal Core will produce mutant mice by microinjecting genetically engineered Embryonic Stem cells into the blastocoel of the embryonic day 3.5 mouse embryos and transgenic mice by DNA microinjection into the male pronucleus of one-cell mouse embryos.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54HD007495-40
Application #
8446126
Study Section
Special Emphasis Panel (ZHD1-DSR-L)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
40
Fiscal Year
2013
Total Cost
$135,380
Indirect Cost
$64,087
Name
Baylor College of Medicine
Department
Type
DUNS #
051113330
City
Houston
State
TX
Country
United States
Zip Code
77030
Lloyd, Richard E (2016) Enterovirus Control of Translation and RNA Granule Stress Responses. Viruses 8:93
Ren, Yi A; Liu, Zhilin; Mullany, Lisa K et al. (2016) Growth Arrest Specific-1 (GAS1) Is a C/EBP Target Gene That Functions in Ovulation and Corpus Luteum Formation in Mice. Biol Reprod 94:44
Toneff, M J; Sreekumar, A; Tinnirello, A et al. (2016) The Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states. BMC Biol 14:47
Trial, JoAnn; Cieslik, Katarzyna A; Entman, Mark L (2016) Phosphocholine-containing ligands direct CRP induction of M2 macrophage polarization independent of T cell polarization: Implication for chronic inflammatory states. Immun Inflamm Dis 4:274-88
Giudice, Jimena; Loehr, James A; Rodney, George G et al. (2016) Alternative Splicing of Four Trafficking Genes Regulates Myofiber Structure and Skeletal Muscle Physiology. Cell Rep 17:1923-1933
Matatall, Katie A; Jeong, Mira; Chen, Siyi et al. (2016) Chronic Infection Depletes Hematopoietic Stem Cells through Stress-Induced Terminal Differentiation. Cell Rep 17:2584-2595
McConnell, Kellie I; Shamsudeen, Sabeel; Meraz, Ismail M et al. (2016) Reduced Cationic Nanoparticle Cytotoxicity Based on Serum Masking of Surface Potential. J Biomed Nanotechnol 12:154-64
Han, Sang Jun; Begum, Khurshida; Foulds, Charles E et al. (2016) The Dual Estrogen Receptor α Inhibitory Effects of the Tissue-Selective Estrogen Complex for Endometrial and Breast Safety. Mol Pharmacol 89:14-26
Huq, Redwan; Samuel, Errol L G; Sikkema, William K A et al. (2016) Preferential uptake of antioxidant carbon nanoparticles by T lymphocytes for immunomodulation. Sci Rep 6:33808
Szafran, Adam T; Mancini, Maureen G; Nickerson, Jeffrey A et al. (2016) Use of HCA in subproteome-immunization and screening of hybridoma supernatants to define distinct antibody binding patterns. Methods 96:75-84

Showing the most recent 10 out of 129 publications