The Integrated Microscopy Core has been an important resource for this U54 Center for Reproductive Biology from its inception over 35 years ago. The Core, enabling routine and sophisticated light microscopy (LM) and electron microscopy (EM), has significantly supported all four Projects in the last funding period, and continues to be an integral support component of the current proposal. The Core has provided LM and EM resources, training and services for a wide range of biological projects. These include the longstanding general imaging of histological and ultrastructural specimens, and over the years has evolved to provide an increasing number of integrated image-based approaches that bring together biochemistry, cell biology, molecular and genetic studies (e.g., interaction, expression levels, immunological, epitope-tagging, reporter gene studies, etc). The most recent technological shift has been to increased implementation of automation and highly quantitative functional and cytological measurements obtained through the installation of high throughput microscopy (HTM) equipment. Effectively, the ability of the Integrated Microscopy Core now provides not only the routine and advanced imaging resource, but is increasingly bringing to the forefront high throughput systems biology as a Research &Development tool to bring new technologies to the U54 Projects. HTM will be critical for the investigators in this U54 application. The focus of the research in this U54 renewal application is the investigation of the role of co-regulators, transcription factors, cell signaling and micro RNA in the regulation of endometrial and ovarian function in the mouse and human. All these approaches will utilize imaging as a tool to evaluate endometrial biology. The execution of the aims of these projects will require the core to be an evolving tool.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54HD007495-40
Application #
8446128
Study Section
Special Emphasis Panel (ZHD1-DSR-L)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
40
Fiscal Year
2013
Total Cost
$142,505
Indirect Cost
$64,087
Name
Baylor College of Medicine
Department
Type
DUNS #
051113330
City
Houston
State
TX
Country
United States
Zip Code
77030
Lloyd, Richard E (2016) Enterovirus Control of Translation and RNA Granule Stress Responses. Viruses 8:93
Ren, Yi A; Liu, Zhilin; Mullany, Lisa K et al. (2016) Growth Arrest Specific-1 (GAS1) Is a C/EBP Target Gene That Functions in Ovulation and Corpus Luteum Formation in Mice. Biol Reprod 94:44
Toneff, M J; Sreekumar, A; Tinnirello, A et al. (2016) The Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states. BMC Biol 14:47
Trial, JoAnn; Cieslik, Katarzyna A; Entman, Mark L (2016) Phosphocholine-containing ligands direct CRP induction of M2 macrophage polarization independent of T cell polarization: Implication for chronic inflammatory states. Immun Inflamm Dis 4:274-88
Giudice, Jimena; Loehr, James A; Rodney, George G et al. (2016) Alternative Splicing of Four Trafficking Genes Regulates Myofiber Structure and Skeletal Muscle Physiology. Cell Rep 17:1923-1933
Matatall, Katie A; Jeong, Mira; Chen, Siyi et al. (2016) Chronic Infection Depletes Hematopoietic Stem Cells through Stress-Induced Terminal Differentiation. Cell Rep 17:2584-2595
McConnell, Kellie I; Shamsudeen, Sabeel; Meraz, Ismail M et al. (2016) Reduced Cationic Nanoparticle Cytotoxicity Based on Serum Masking of Surface Potential. J Biomed Nanotechnol 12:154-64
Han, Sang Jun; Begum, Khurshida; Foulds, Charles E et al. (2016) The Dual Estrogen Receptor α Inhibitory Effects of the Tissue-Selective Estrogen Complex for Endometrial and Breast Safety. Mol Pharmacol 89:14-26
Huq, Redwan; Samuel, Errol L G; Sikkema, William K A et al. (2016) Preferential uptake of antioxidant carbon nanoparticles by T lymphocytes for immunomodulation. Sci Rep 6:33808
Szafran, Adam T; Mancini, Maureen G; Nickerson, Jeffrey A et al. (2016) Use of HCA in subproteome-immunization and screening of hybridoma supernatants to define distinct antibody binding patterns. Methods 96:75-84

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