The mission of the Specialized Cooperative Centers Program in Reproduction Research (SCCPRR) at the University of Pittsburgh School of Medicine continues to be the study of the physiology (integrative genomics) of the control systems that govern gonadal function in higher primates, including man. This mission of the Pittsburgh SCCPRR, which reflects the long-standing forte of this Center to employ non-human primate models to better understand human reproduction, is pursued by integrating molecular, cellular and system approaches. The Center will be comprised of two Technical Service Cores (Primate and Assay) with an open access format to sub serve six SCCPRR projects (four in Pittsburgh), three projects at the Cooperative Reproductive Sciences Center at Morehouse School of Medicine, and several other programs supported by either R01 or R21 grants. Three of the four Pittsburgh SCCPRR projects focus on the development and control of the testes, with a particular emphasis on the regulation of proliferation and differentiation of stem spermatogonia (male germ-line stem cells, GSCs) and their niche providing somatic cells of Sertoli. Studies of premeiotic spermatogonia will be restricted to the human and monkey testes, and although the molecular biology underlying Sertoli cell proliferation and differentiation will be elucidated with non-primate models, we aim to concomitantly translate findings in the rodent to the monkey. The fourth project will examine the control mechanisms that govern the early stages of follicular development using a non-human primate model. Both conceptual and methodological bridges link the four projects. In this regard, there are parallels in the paracrine control of follicular development, on the one hand, and the regulation of the male germinal epithelium, on the other. At the technical level, methodology developed at the Pittsburgh Center for controlling gene expression in granulosa cells will find direct application to the study of Sertoli cell biology in this proposal. The theme of the primate gonad is explored with vertical balance in investigator experience, and it is anticipated that this approach will guarantee that the Pittsburgh SCCPRR will continue to contribute significantly to our understanding of human reproduction and to the treatment of its disorders.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54HD008610-35
Application #
7843442
Study Section
Special Emphasis Panel (ZHD1-DRG-D (04))
Program Officer
De Paolo, Louis V
Project Start
1997-04-01
Project End
2013-03-31
Budget Start
2010-04-01
Budget End
2013-03-31
Support Year
35
Fiscal Year
2010
Total Cost
$1,067,137
Indirect Cost
Name
University of Pittsburgh
Department
Physiology
Type
Schools of Medicine
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Vargas Trujillo, Marcela; Kalil, Bruna; Ramaswamy, Suresh et al. (2017) Estradiol Upregulates Kisspeptin Expression in the Preoptic Area of both the Male and Female Rhesus Monkey (Macaca mulatta): Implications for the Hypothalamic Control of Ovulation in Highly Evolved Primates. Neuroendocrinology 105:77-89
Kalil, Bruna; Ramaswamy, Suresh; Plant, Tony M (2016) The Distribution of Substance P and Kisspeptin in the Mediobasal Hypothalamus of the Male Rhesus Monkey and a Comparison of Intravenous Administration of These Peptides to Release GnRH as Reflected by LH Secretion. Neuroendocrinology 103:711-23
Walker, William H; Easton, Evan; Moreci, Rebecca S et al. (2015) Restoration of spermatogenesis and male fertility using an androgen receptor transgene. PLoS One 10:e0120783
Lomniczi, Alejandro; Wright, Hollis; Castellano, Juan Manuel et al. (2015) Epigenetic regulation of puberty via Zinc finger protein-mediated transcriptional repression. Nat Commun 6:10195
Shahab, M; Trujillo, M Vargas; Plant, T M (2015) A Reevaluation of the Question: Is the Pubertal Resurgence in Pulsatile GnRH Release in the Male Rhesus Monkey (Macaca mulatta) Associated With a Gonad-Independent Augmentation of GH Secretion? Endocrinology 156:3717-24
Verhagen, I; Ramaswamy, S; Teerds, K J et al. (2014) Time course and role of luteinizing hormone and follicle-stimulating hormone in the expansion of the Leydig cell population at the time of puberty in the rhesus monkey (Macaca mulatta). Andrology 2:924-30
Ramaswamy, Suresh; Dwarki, Karthik; Ali, Barkat et al. (2013) The decline in pulsatile GnRH release, as reflected by circulating LH concentrations, during the infant-juvenile transition in the agonadal male rhesus monkey (Macaca mulatta) is associated with a reduction in kisspeptin content of KNDy neurons of the arc Endocrinology 154:1845-53
Terasawa, Ei; Guerriero, Kathryn A; Plant, Tony M (2013) Kisspeptin and puberty in mammals. Adv Exp Med Biol 784:253-73
Stephens, Sahar M; Pau, Francis K Y; Yalcinkaya, Tamer M et al. (2013) Assessing the pulsatility of luteinizing hormone in female vervet monkeys (Chlorocebus aethiops sabaeus). Comp Med 63:432-8
Jahnukainen, Kirsi; Ehmcke, Jens; Nurmio, Mirja et al. (2012) Autologous ectopic grafting of cryopreserved testicular tissue preserves the fertility of prepubescent monkeys that receive sterilizing cytotoxic therapy. Cancer Res 72:5174-8

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