As a National RSB Resource, the Ligand Assay and Analysis Core will serve an essential role in this U54 Center, and other RSB-supported Centers. First, the Core will provide high quality, cost effective assay services. Second, develop new state-of-the-art assay methods to accommodate investigator needs. Third, the Core will provide assistance and consultation regarding assay methodology, data reduction/analysis, data interpretation and biomathematical modeling. The accurate, precise and dependable measurement of reproductive hormones is an essential component in the mission of Ligand Core. The justification for establishing a Core facility relates to the savings in cost, resources and personnel to internal and external RSB-supported investigators, as they will not need to develop and maintain these methods in each individual laboratory. The technical expertise and equipment required to perform high volume or diverse testing by each investigator would be inefficient and divert effort and resources from important research questions. Also, a central Core facility can implement uniform standards for assay performance, quality controls and data reduction. The implementation of new methods or enhancement of current methods require technical expertise that would be costly and inefficient to establish in-house by each project investigator. One of the major rationales for establishing the Core is to provide state of the art assay services in a cost efficient manner. This mission will include development of assays with enhanced sensitivity and specificity, transferring methods from radioactive to nonradioactive systems, implementation of automation equipment (for high volume tests). Assay automation can enhance assay performance by improving assay precision and reducing costs by increasing the number of tests that can be performed by the technologist. Finally, providing services to other RSB-supported Centers and other reproductive scientists within the University of Virginia, promote a spirit of cooperation that may lead to future collaborations between investigators.

Public Health Relevance

The Ligand Core will perform state of the art assay services for clinical and basic scientific investigations of reproduction. Approximately half of the assays performed by the Core are derived from clinical research projects (from this U54 Center and other RSB-supported Centers). Most of these clinical projects are investigating important aspects of human infertility, and the accurate measurement of critical reproductive hormones is essential. PROJECT/PERFORMANCE SITE(S) (if additional spac

National Institute of Health (NIH)
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Specialized Center--Cooperative Agreements (U54)
Project #
Application #
Study Section
Special Emphasis Panel (ZHD1-DSR-L)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Virginia
United States
Zip Code
Bu, Pengli; Yagi, Shintaro; Shiota, Kunio et al. (2017) Origin of a rapidly evolving homeostatic control system programming testis function. J Endocrinol 234:217-232
Gorsic, Lidija K; Kosova, Gulum; Werstein, Brian et al. (2017) Pathogenic Anti-Müllerian Hormone Variants in Polycystic Ovary Syndrome. J Clin Endocrinol Metab 102:2862-2872
Prins, Gail S; Ye, Shu-Hua; Birch, Lynn et al. (2017) Prostate Cancer Risk and DNA Methylation Signatures in Aging Rats following Developmental BPA Exposure: A Dose-Response Analysis. Environ Health Perspect 125:077007
Sam, Susan; Vellanki, Priyathama; Yalamanchi, Sudha K et al. (2017) Exaggerated glucagon responses to hypoglycemia in women with polycystic ovary syndrome. Metabolism 71:125-131
Clark, Nicole C; Pru, Cindy A; Yee, Siu-Pok et al. (2017) Conditional Ablation of Progesterone Receptor Membrane Component 2 Causes Female Premature Reproductive Senescence. Endocrinology 158:640-651
Jonak, Carrie R; Lainez, Nancy M; Roybal, Lacey L et al. (2017) c-JUN Dimerization Protein 2 (JDP2) Is a Transcriptional Repressor of Follicle-stimulating Hormone ? (FSH?) and Is Required for Preventing Premature Reproductive Senescence in Female Mice. J Biol Chem 292:2646-2659
Deckard, Charles; Walker, Azida; Hill, Brent J F (2017) Using three-point bending to evaluate tibia bone strength in ovariectomized young mice. J Biol Phys 43:139-148
Levasseur, Adrien; St-Jean, Guillaume; Paquet, Marilène et al. (2017) Targeted Disruption of YAP and TAZ Impairs the Maintenance of the Adrenal Cortex. Endocrinology 158:3738-3753
Scalici, Jennifer M; Arapovic, Sanja; Saks, Erin J et al. (2017) Mesothelium expression of vascular cell adhesion molecule-1 (VCAM-1) is associated with an unfavorable prognosis in epithelial ovarian cancer (EOC). Cancer 123:977-984
Reid, Sara Pittenger; Kao, Chia-Ning; Pasch, Lauri et al. (2017) Ovarian morphology is associated with insulin resistance in women with polycystic ovary syndrome: a cross sectional study. Fertil Res Pract 3:8

Showing the most recent 10 out of 446 publications