The Neonatal Translational Core is located in at SUNY Downstate Medical Center. The key functional capabilities are: a) animal models for oxygen-induced retinopathy;2) cell culture models for oxidative stress in the retina;c) biochemical analyses;d) gene profiling;and e) immunohistochemistry, immunofluorescence and imaging analyses.
The aims of this core are to promote and foster collaborative research interactions among PIs;establish state-of-the-art translational research training core facility focusing on oxygen-induced retinopathy and oxidative stress for junior faculty, fellows, residents, and research scientists;and recruit basic and clinical investigators into retinopathy research. Accessibility to this Core will be afforded to support the ongoing and newly emerging research programs covered in this proposal, which requires the kinds of methodologies and analytical capabilities offered by this core. This core will also facilitate ongoing and new collaborations between investigators who are developing novel therapeutic efforts for Retinopathy of Prematurity. Each of these services has been extensively used by the Pediatric PIs and there is great demand for these services. Many of the services and/or use of equipment will be free of charge to investigators;these include all molecular services;biochemical services;use of the equipment. In order to access services the following service order forms will be available. For Protocol 1, this core will be responsible for all experimental phases including animal handling, drug administration, hyperoxia/hypoxia cycling, blood and tissue harvesting, sample processing and image analyses in addition to the other cores. For Protocol 2, this core will be responsible for all experimental phases including plating the cells, SiRNA interference, media and cell harvesting, sample processing and image analyses in addition to the other cores. For Protocol 3, the neonatal translational core will analyze all patient blood samples prostaglandins, VEGF, sVEGFR-1, IGF-I and gene profiling of VEGF and Notch signaling in the placenta, cord and blood samples.
|Nouri-Nigjeh, Eslam; Sukumaran, Siddharth; Tu, Chengjian et al. (2014) Highly multiplexed and reproducible ion-current-based strategy for large-scale quantitative proteomics and the application to protein expression dynamics induced by methylprednisolone in 60 rats. Anal Chem 86:8149-57|
|Tu, Chengjian; Mammen, Manoj Jacob; Li, Jun et al. (2014) Large-scale, ion-current-based proteomics investigation of bronchoalveolar lavage fluid in chronic obstructive pulmonary disease patients. J Proteome Res 13:627-39|
|An, Bo; Zhang, Ming; Qu, Jun (2014) Toward sensitive and accurate analysis of antibody biotherapeutics by liquid chromatography coupled with mass spectrometry. Drug Metab Dispos 42:1858-66|
|Tu, Chengjian; Li, Jun; Sheng, Quanhu et al. (2014) Systematic assessment of survey scan and MS2-based abundance strategies for label-free quantitative proteomics using high-resolution MS data. J Proteome Res 13:2069-79|
|Nouri-Nigjeh, Eslam; Zhang, Ming; Ji, Tao et al. (2014) Effects of calibration approaches on the accuracy for LC-MS targeted quantification of therapeutic protein. Anal Chem 86:3575-84|
|Qu, Jun; Young, Rebeccah; Page, Brian J et al. (2014) Reproducible ion-current-based approach for 24-plex comparison of the tissue proteomes of hibernating versus normal myocardium in swine models. J Proteome Res 13:2571-84|
|Gui, Shanying; Gathiaka, Symon; Li, Jun et al. (2014) A remodeled protein arginine methyltransferase 1 (PRMT1) generates symmetric dimethylarginine. J Biol Chem 289:9320-7|
|Shen, Xiaomeng; Young, Rebeccah; Canty, John M et al. (2014) Quantitative proteomics in cardiovascular research: global and targeted strategies. Proteomics Clin Appl 8:488-505|
|Chen, Ting; Mager, Donald E; Kagan, Leonid (2013) Interspecies modeling and prediction of human exenatide pharmacokinetics. Pharm Res 30:751-60|
|Beharry, Kay D; Cai, Charles L; Sharma, Poonam et al. (2013) Hydrogen peroxide accumulation in the choroid during intermittent hypoxia increases risk of severe oxygen-induced retinopathy in neonatal rats. Invest Ophthalmol Vis Sci 54:7644-57|
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