The Neonatal Translational Core is located in at SUNY Downstate Medical Center. The key functional capabilities are: a) animal models for oxygen-induced retinopathy;2) cell culture models for oxidative stress in the retina;c) biochemical analyses;d) gene profiling;and e) immunohistochemistry, immunofluorescence and imaging analyses.
The aims of this core are to promote and foster collaborative research interactions among PIs;establish state-of-the-art translational research training core facility focusing on oxygen-induced retinopathy and oxidative stress for junior faculty, fellows, residents, and research scientists;and recruit basic and clinical investigators into retinopathy research. Accessibility to this Core will be afforded to support the ongoing and newly emerging research programs covered in this proposal, which requires the kinds of methodologies and analytical capabilities offered by this core. This core will also facilitate ongoing and new collaborations between investigators who are developing novel therapeutic efforts for Retinopathy of Prematurity. Each of these services has been extensively used by the Pediatric PIs and there is great demand for these services. Many of the services and/or use of equipment will be free of charge to investigators;these include all molecular services;biochemical services;use of the equipment. In order to access services the following service order forms will be available. For Protocol 1, this core will be responsible for all experimental phases including animal handling, drug administration, hyperoxia/hypoxia cycling, blood and tissue harvesting, sample processing and image analyses in addition to the other cores. For Protocol 2, this core will be responsible for all experimental phases including plating the cells, SiRNA interference, media and cell harvesting, sample processing and image analyses in addition to the other cores. For Protocol 3, the neonatal translational core will analyze all patient blood samples prostaglandins, VEGF, sVEGFR-1, IGF-I and gene profiling of VEGF and Notch signaling in the placenta, cord and blood samples.
|Tu, Chengjian; Li, Jun; Shen, Shichen et al. (2016) Performance Investigation of Proteomic Identification by HCD/CID Fragmentations in Combination with High/Low-Resolution Detectors on a Tribrid, High-Field Orbitrap Instrument. PLoS One 11:e0160160|
|Beharry, Kay D; Valencia, Gloria B; Lazzaro, Douglas R et al. (2016) Pharmacologic interventions for the prevention and treatment of retinopathy of prematurity. Semin Perinatol 40:189-202|
|Inayat, Musaddaq; Bany-Mohammed, Fayez; Valencia, Arwin et al. (2015) Antioxidants and Biomarkers of Oxidative Stress in Preterm Infants with Symptomatic Patent Ductus Arteriosus. Am J Perinatol 32:895-904|
|An, Bo; Zhang, Ming; Johnson, Robert W et al. (2015) Surfactant-aided precipitation/on-pellet-digestion (SOD) procedure provides robust and rapid sample preparation for reproducible, accurate and sensitive LC/MS quantification of therapeutic protein in plasma and tissues. Anal Chem 87:4023-9|
|Shen, Xiaomeng; Nair, Bindukumar; Mahajan, Supriya D et al. (2015) New Insights into the Disease Progression Control Mechanisms by Comparing Long-Term-Nonprogressors versus Normal-Progressors among HIV-1-Positive Patients Using an Ion Current-Based MS1 Proteomic Profiling. J Proteome Res 14:5225-39|
|Jivabhai Patel, Shamin; Bany-Mohammed, Fayez; McNally, Lois et al. (2015) Exogenous Superoxide Dismutase Mimetic Without Scavenging H2O2 Causes Photoreceptor Damage in a Rat Model for Oxygen-Induced Retinopathy. Invest Ophthalmol Vis Sci 56:1665-77|
|Tu, Chengjian; Beharry, Kay D; Shen, Xiaomeng et al. (2015) Proteomic profiling of the retinas in a neonatal rat model of oxygen-induced retinopathy with a reproducible ion-current-based MS1 approach. J Proteome Res 14:2109-20|
|Tu, Chengjian; Sheng, Quanhu; Li, Jun et al. (2015) Optimization of Search Engines and Postprocessing Approaches to Maximize Peptide and Protein Identification for High-Resolution Mass Data. J Proteome Res 14:4662-73|
|Kamisoglu, Kubra; Sukumaran, Siddharth; Nouri-Nigjeh, Eslam et al. (2015) Tandem analysis of transcriptome and proteome changes after a single dose of corticosteroid: a systems approach to liver function in pharmacogenomics. OMICS 19:80-91|
|Lott, Kaylen; Mukhopadhyay, Shreya; Li, Jun et al. (2015) Arginine methylation of DRBD18 differentially impacts its opposing effects on the trypanosome transcriptome. Nucleic Acids Res 43:5501-23|
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