The Nonhuman Primate Core (NHP Core) will function as a "closed core" to provide NHP resources and technical support for the projects proposed in this SCCPIR Center. These projects are: Project I, Metabolic &Neuroendocrine Function (Grove and Cameron co-PIs);Project II, Assessment of Reproductive Parameters (Stouffer, PI);Project III, Adipose Metabolism (Roberts, PI). The broad goal of this SCCPIR Center is to assess the independent and combined effects of testosterone (T) and a high calorie diet (typical of the "Western-style diet";WSD) on reproductive function. The specific goal of the NHP Core is to provide significant cost savings to the SCCPIR Center projects. The NHP Core will lease and maintain 48 female rhesus monkeys for study by Projects l-lll. This will eliminate repeated lease fees and setup charges for the individual projects. The NHP Core will coordinate veterinary care and husbandry of the animals through the Oregon National Primate Research Center (ONPRC), Division of Animal Resources (DAR). The NHP Core will administer the WSD, thereby reducing per diem charges associated with the preparation of special diets. The NHP Core will provide a dedicated set of technical staff and management that would otherwise be cost prohibitive for studies of this size. The NHP Core staff is trained to perform routine surgical and other procedures (e.g. T implant placement) that would otherwise require scheduling with the DAR Surgical Unit, greatly saving on procedural costs. There will be no fee structure or chargebacks for Projects l-lll. The NHP Core will provide general supplies (syringes, blood tubes, storage vials, swabs, etc.) leaving individual investigators responsible only for costs for procedures/assays unique to their particular projects. Researchers will plan studies with the NHP Core during regularly scheduled meetings between Dr. Slayden, the core supervisor, and NHP Core staff. NHP Core oversight will be provided by an Oversight Committee that will consist of Dr. Charles T. Roberts PhD, Associate Director ONPRC (Chair);Judy L. Cameron PhD, Affiliate Scientist ONPRC;Chris D. Kroenke PhD;Jon D. Hennebold PhD, Associate Scientist, ONPRC Division of Reproductive Sciences;Theodore R Hobbs DVM, Associate Veterinarian, Head ONPRC DAR Surgery Unit.

Public Health Relevance

Obesity and androgen excess are frequent conditions reported for pre-pubertal girls at risk for polycystic ovarian syndrome. The goal of this SCCPIR Center is to investigate the role of high calorie diet and androgen excess on reproductive function. The goal of the NHP Core is to reduce costs to this SCCPIR Center through sharing of animal resources and services.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
1U54HD071836-01A1
Application #
8510101
Study Section
Special Emphasis Panel (ZHD1-DSR-L (55))
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
1
Fiscal Year
2013
Total Cost
$99,655
Indirect Cost
$34,944
Name
Oregon Health and Science University
Department
Type
DUNS #
096997515
City
Portland
State
OR
Country
United States
Zip Code
97239
Keller, Erica; Chazenbalk, Gregorio D; Aguilera, Paul et al. (2014) Impaired preadipocyte differentiation into adipocytes in subcutaneous abdominal adipose of PCOS-like female rhesus monkeys. Endocrinology 155:2696-703
McGee, W K; Bishop, C V; Pohl, C R et al. (2014) Effects of hyperandrogenemia and increased adiposity on reproductive and metabolic parameters in young adult female monkeys. Am J Physiol Endocrinol Metab 306:E1292-304
Amin, Marli; Simerman, Ariel; Cho, Michele et al. (2014) 21-Hydroxylase-derived steroids in follicles of nonobese women undergoing ovarian stimulation for in vitro fertilization (IVF) positively correlate with lipid content of luteinized granulosa cells (LGCs) as a source of cholesterol for steroid synthesis. J Clin Endocrinol Metab 99:1299-306
Simerman, Ariel A; Perone, Marcelo J; Gimeno, MarĂ­a L et al. (2014) A mystery unraveled: nontumorigenic pluripotent stem cells in human adult tissues. Expert Opin Biol Ther 14:917-29
Dumesic, Daniel A; Goodarzi, Mark O; Chazenbalk, Gregorio D et al. (2014) Intrauterine environment and polycystic ovary syndrome. Semin Reprod Med 32:159-65
Varlamov, Oleg; Chu, Michael P; McGee, Whitney K et al. (2013) Ovarian cycle-specific regulation of adipose tissue lipid storage by testosterone in female nonhuman primates. Endocrinology 154:4126-35
Chazenbalk, Gregorio; Singh, Prapti; Irge, Dana et al. (2013) Androgens inhibit adipogenesis during human adipose stem cell commitment to preadipocyte formation. Steroids 78:920-6
Heneidi, Saleh; Simerman, Ariel A; Keller, Erica et al. (2013) Awakened by cellular stress: isolation and characterization of a novel population of pluripotent stem cells derived from human adipose tissue. PLoS One 8:e64752