Abstract

Specific Aims: The goal of this research is to better understand the metabolic state of cells that are deprived of oxygen. In this initial pilot project phase we will adapt methods for analyzing metabolism energetics and oxidative damage to studying the model organism zebrafish (Danio rerio). We will apply these methods: 1. To determine the change of the metabolism and intracellular energetics of zebrafish embryos to varying oxygen tensions at different periods in development. 2. To define the extent of oxidative damage that results from alterations in the oxygen levels. 3. To develop and begin to test hypotheses about mechanisms of surviving hypoxia that may be common to zebrafish and tumors.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Exploratory Grants--Cooperative Agreements (U56)
Project #
1U56CA096288-01
Application #
6606739
Study Section
Special Emphasis Panel (ZCA1)
Project Start
2002-06-12
Project End
2007-05-31
Budget Start
Budget End
Support Year
1
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
075524595
City
Seattle
State
WA
Country
United States
Zip Code
98109

  Publications

Thompson, Beti; O'Connell, Mary; Löest, Helena et al. (2013) Understanding and reducing obstacles in a collaboration between a minority institution and a cancer center. J Health Care Poor Underserved 24:1648-56
Shuster, Michele; Peterson, Karen (2009) Development, implementation, and assessment of a lecture course on cancer for undergraduates. CBE Life Sci Educ 8:193-202
O'Keefe, David D; Gonzalez-Niño, Eduardo; Burnett, Micheal et al. (2009) Rap1 maintains adhesion between cells to affect Egfr signaling and planar cell polarity in Drosophila. Dev Biol 333:143-60
O'Keefe, David D; Prober, David A; Moyle, Patrick S et al. (2007) Egfr/Ras signaling regulates DE-cadherin/Shotgun localization to control vein morphogenesis in the Drosophila wing. Dev Biol 311:25-39
Coronado, Gloria D; Farias, Argelia; Thompson, Beti et al. (2006) Attitudes and beliefs about colorectal cancer among Mexican Americans in communities along the US-Mexico border. Ethn Dis 16:421-7
George, Olivia; Johnston, Mantissa A; Shuster, Charles B (2006) Aurora B kinase maintains chromatin organization during the MI to MII transition in surf clam oocytes. Cell Cycle 5:2648-56
Li, C I; Uribe, D J; Daling, J R (2005) Clinical characteristics of different histologic types of breast cancer. Br J Cancer 93:1046-52