Malaria Vaccine Development Branch (MVDB)

Saul, Allan

Abstract

Malaria Vaccine Development Branch (MVDB) General Description: The Malaria Vaccine Development Unit (MVDU) is an NIAID initiative working in close collaboration with DMID to respond to the global need for vaccines against malaria. ? ? We now have produced eight cGMP recombinant protein products: AMA1-FVO, AMA1-3D7, AMA1-L32, MSP1(42)-FVO, MSP1(42)-3D7, MSP1(42)-FUP, Pfs25, Pvs25. Six of these antigens (Pvs25H, Pfs25, AMA1-FVO, AMA1-3D7, MSP1(42)-FVO and MSP1(42)-3D7) are in a series of human Phase 1 and Phase 2 vaccine trials, either alone or in combinations using three anjuvants (Aluminum hydroxide, Montanide ISA51 and Aluminum hydroxide with CPG7909). The Phase 1 trials are underway in the USA and Phase 1 and Phase 2 trials are underway in an endemic population in Mali in collaboration with the University of Bamako, Mali. These vaccine trials form part of a clinical development plan working towards Phase 2 trials in an endemic area that will determine if these vaccines are able to make a substantial impact on parasite growth or transmission.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Intramural Research (Z01)
• Project #: 1Z01AI000727-12
• Application #: 7302229
• Study Section: (LPD)
• Support Year: 12
• Fiscal Year: 2006

Institution

• Name: Niaid Extramural Activities
• Country: United States

Publications

Shimp Jr, Richard L; Martin, Laura B; Zhang, Yanling et al. (2006) Production and characterization of clinical grade Escherichia coli derived Plasmodium falciparum 42 kDa merozoite surface protein 1 (MSP1(42)) in the absence of an affinity tag. Protein Expr Purif 50:58-67

Singh, Sanjay; Miura, Kazutoyo; Zhou, Hong et al. (2006) Immunity to recombinant plasmodium falciparum merozoite surface protein 1 (MSP1): protection in Aotus nancymai monkeys strongly correlates with anti-MSP1 antibody titer and in vitro parasite-inhibitory activity. Infect Immun 74:4573-80

Collins, William E; Barnwell, John W; Sullivan, Joann S et al. (2006) Assessment of transmission-blocking activity of candidate Pvs25 vaccine using gametocytes from chimpanzees. Am J Trop Med Hyg 74:215-21

Tsai, Chiawei W; Duggan, Peter F; Shimp Jr, Richard L et al. (2006) Overproduction of Pichia pastoris or Plasmodium falciparum protein disulfide isomerase affects expression, folding and O-linked glycosylation of a malaria vaccine candidate expressed in P. pastoris. J Biotechnol 121:458-70

Makobongo, Morris O; Keegan, Brian; Long, Carole A et al. (2006) Immunization of Aotus monkeys with recombinant cysteine-rich interdomain region 1 alpha protects against severe disease during Plasmodium falciparum reinfection. J Infect Dis 193:731-40

Wille-Reece, Ulrike; Flynn, Barbara J; Lore, Karin et al. (2006) Toll-like receptor agonists influence the magnitude and quality of memory T cell responses after prime-boost immunization in nonhuman primates. J Exp Med 203:1249-58

Giersing, Birgitte K; Dubovsky, Filip; Saul, Allan et al. (2006) Potency assay design for adjuvanted recombinant proteins as malaria vaccines. Vaccine 24:4264-70

Trinh, Loc; Phue, Je-Nie; Jaluria, Pratik et al. (2006) Screen-less expanded bed column: new approach for the recovery and purification of a malaria transmission blocking vaccine candidate from Pichia pastoris. Biotechnol Lett 28:951-8

Saxena, Ajay K; Singh, Kavita; Su, Hua-Poo et al. (2006) The essential mosquito-stage P25 and P28 proteins from Plasmodium form tile-like triangular prisms. Nat Struct Mol Biol 13:90-1

Mullen, Gregory E D; Giersing, Birgitte K; Ajose-Popoola, Olubunmi et al. (2006) Enhancement of functional antibody responses to AMA1-C1/Alhydrogel, a Plasmodium falciparum malaria vaccine, with CpG oligodeoxynucleotide. Vaccine 24:2497-505