Proteolysis of von Willebrand factor (VWF) normally occurs through the action of a plasma enzyme that has recently been characterized (ADAMTS13); it accounts for the small quantities of cleavage products normally present in the circulation, and its inhibition can lead to the disease called thrombotic thrombocytopenic purpura (TTP). We developed a rapid assay to evaluate the cleavage of VWF and have characterized patients with a TTP-like syndrome to detect those with low VWF cleaving protease activity. The assay does not require specialized reagents and can be completed within 6-8 hours on patient plasma. We participated in an international study testing coded samples with varying levels of ADAMTS13 to compare our method with other more quantitative methods and found that we could differentiate normal from abnormal values in 95% of samples. We also studied a specific patient in detail who had SLE and an episode of TTP in whom the diagnosis was problematic due to the underlying disease. The level of protease activity and its inhibitor aided in the diagnosis and clinical management, including the duration of plasma exchange, in this patient. The assay has also been used to follow treatment of TTP patients using rituximab. A new assay using a flow chamber, rather than a static system, to assess the level of enzyme activity is under development.

Agency
National Institute of Health (NIH)
Institute
Clinical Center (CLC)
Type
Intramural Research (Z01)
Project #
1Z01CL010300-07
Application #
7215825
Study Section
(DLM)
Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
2005
Total Cost
Indirect Cost
Name
Clinical Center
Department
Type
DUNS #
City
State
Country
United States
Zip Code