Abstract

A partial clone of the prostaglandin synthase I gene from mouse strain 129 genomic DNA was obtained. A targeting construct was made using 7.5kb of the genomic clone with an inserted bacterial neo gene and trailing herpes simplex virus TK gene. The construct was electroporated into embryonic stem cells and G418/gangcyclovir resistant clones were isolated. Homologously targeted ES cells were identified by Southern and PCR analysis. The targeted ES cells with a single allele of the PHS-1 disrupted have been injected into bastocysts and the blastocysts implanted. The births of chimeric and, subsequently, germline transmitting animals are awaited. These animals will be utilized to determine the role of PHS-1 in carcinogenesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Intramural Research (Z01)
Project #
1Z01ES021179-01
Application #
3755386
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
National Institute of Environmental Health Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Aid, Saba; Langenbach, Robert; Bosetti, Francesca (2008) Neuroinflammatory response to lipopolysaccharide is exacerbated in mice genetically deficient in cyclooxygenase-2. J Neuroinflammation 5:17
Choi, Sang-Ho; Langenbach, Robert; Bosetti, Francesca (2008) Genetic deletion or pharmacological inhibition of cyclooxygenase-1 attenuate lipopolysaccharide-induced inflammatory response and brain injury. FASEB J 22:1491-501
Narasimha, Ajay; Watanabe, Junji; Lin, James A et al. (2007) A novel anti-atherogenic role for COX-2--potential mechanism for the cardiovascular side effects of COX-2 inhibitors. Prostaglandins Other Lipid Mediat 84:24-33
Gitlin, Jonathan M; Trivedi, Darshini B; Langenbach, Robert et al. (2007) Genetic deficiency of cyclooxygenase-2 attenuates abdominal aortic aneurysm formation in mice. Cardiovasc Res 73:227-36
Carey, Michelle A; Bradbury, J Alyce; Seubert, John M et al. (2005) Contrasting effects of cyclooxygenase-1 (COX-1) and COX-2 deficiency on the host response to influenza A viral infection. J Immunol 175:6878-84
Rao, Jagadeesh S; Langenbach, Robert; Bosetti, Francesca (2005) Down-regulation of brain nuclear factor-kappa B pathway in the cyclooxygenase-2 knockout mouse. Brain Res Mol Brain Res 139:217-24
Kawada, Noritaka; Solis, Glenn; Ivey, Nathan et al. (2005) Cyclooxygenase-1-deficient mice have high sleep-to-wake blood pressure ratios and renal vasoconstriction. Hypertension 45:1131-8
Wang, Tongguang; Pei, Zhong; Zhang, Wei et al. (2005) MPP+-induced COX-2 activation and subsequent dopaminergic neurodegeneration. FASEB J 19:1134-6
Steiner, Alexandre A; Rudaya, Alla Y; Robbins, Jared R et al. (2005) Expanding the febrigenic role of cyclooxygenase-2 to the previously overlooked responses. Am J Physiol Regul Integr Comp Physiol 289:R1253-7
Salvatore, Michael F; Fisher, Brent; Surgener, Stewart P et al. (2005) Neurochemical investigations of dopamine neuronal systems in iron-regulatory protein 2 (IRP-2) knockout mice. Brain Res Mol Brain Res 139:341-7

Showing the most recent 10 out of 19 publications