Abstract

of Work: Lactoferrin gene that encodes a noneheme iron-binding glycoprotein serves as an ideal model to study the gene regulation at the molecular level. It is well documented that lactoferrin plays an important role in host defense, inflammatory response and immuno- modulation. The protein is expressed in a variety of tissues and biological fluids under different regulatory mechanisms. The lactoferrin gene is sensitive to estrogens; exnoestrogens and catecholestrogens, retinoic acid; glucocorticoid; forskalin and EGF. Therefore, environmental chemicals which perturb these signaling pathways could affect lactoferrin expression. The two transcription factors, ERRa1 and IKLF, isolated during the course of our present study, are involved in estrogen and EGF regulation of the lactoferrin gene expression, respectively. Characterization of these transcription factors is one step toward understanding the molecular events that are associated with the estrogen and EGF signalling pathways. - promoter, response elements, ERRa1, IKLF, mammalian two-hybrid system, HEC-1B and Jurkat cells

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Intramural Research (Z01)
Project #
1Z01ES070067-17
Application #
6290060
Study Section
Special Emphasis Panel (LRDT)
Project Start
Project End
Budget Start
Budget End
Support Year
17
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
National Institute of Environmental Health Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Teng, Christina T; Li, Yin; Stockton, Pat et al. (2011) Fasting induces the expression of PGC-1? and ERR isoforms in the outer stripe of the outer medulla (OSOM) of the mouse kidney. PLoS One 6:e26961
Li, Yin; Birnbaumer, Lutz; Teng, Christina T (2010) Regulation of ERRalpha gene expression by estrogen receptor agonists and antagonists in SKBR3 breast cancer cells: differential molecular mechanisms mediated by g protein-coupled receptor GPR30/GPER-1. Mol Endocrinol 24:969-80
Li, Yin; Limmon, Gino V; Imani, Farhad et al. (2009) Induction of lactoferrin gene expression by innate immune stimuli in mouse mammary epithelial HC-11 cells. Biochimie 91:58-67
Wang, Liangli; Li, Yin; Hu, Peng et al. (2008) PGC-1 alpha induces dynamic protein interactions on the ERR alpha gene multi-hormone response element nucleosome in kidney cells. Biochem J :
Hu, Peng; Kinyamu, H Karimi; Wang, Liangli et al. (2008) Estrogen induces estrogen-related receptor alpha gene expression and chromatin structural changes in estrogen receptor (ER)-positive and ER-negative breast cancer cells. J Biol Chem 283:6752-63
Zhang, Zhiping; Teng, Christina T (2007) Interplay between estrogen-related receptor alpha (ERRalpha) and gamma (ERRgamma) on the regulation of ERRalpha gene expression. Mol Cell Endocrinol 264:128-41
Teng, Christina T; Gladwell, Wesley (2006) Single nucleotide polymorphisms (SNPs) in human lactoferrin gene. Biochem Cell Biol 84:381-4
Teng, Christina T (2006) Factors regulating lactoferrin gene expression. Biochem Cell Biol 84:263-7
Zhang, Zhiping; Chen, Kevin; Shih, Jean C et al. (2006) Estrogen-related receptors-stimulated monoamine oxidase B promoter activity is down-regulated by estrogen receptors. Mol Endocrinol 20:1547-61
Goldberg, Gary S; Kunimoto, Takehiko; Alexander, David B et al. (2005) Full length and delta lactoferrin display differential cell localization dynamics, but do not act as tumor markers or significantly affect the expression of other genes. Med Chem 1:57-64

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