of work: This project focuses on the mechanism involved in regulating the binding of oxygen to hemoglobin and the transport of oxygen to the tissues. Emphasis is placed on ways in which these functions are impaired and change with age. These studies have focused on the oxidation of hemoglobin, which produces nonfunctional hemoglobin and the simultaneous release of oxyradicals. The enhancement of these oxidative processes under hypoxic conditions is being explored as a possible source of tissue and organ damage, which would be exacerbated during aging. Studies are also included which are directed at the stability of the entire erythrocyte and the erythrocyte membrane. Animal and human studies are performed to investigate possible functional impairment associated with red cell damage.The interaction of amyloids with the erythrocyte is being investigated to determine whether the red cell plays a role in the transport of amyloids and in amyloid induced oxidative stress.

National Institute of Health (NIH)
National Institute on Aging (NIA)
Investigator-Initiated Intramural Research Projects (ZIA)
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National Institute on Aging
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Patel, Kushang V; Mohanty, Joy G; Kanapuru, Bindu et al. (2013) Association of the red cell distribution width with red blood cell deformability. Adv Exp Med Biol 765:211-6
Rajadas, Jayakumar; Sun, Wenchao; Li, Hai et al. (2013) Enhanced A?(1-40) production in endothelial cells stimulated with fibrillar A?(1-42). PLoS One 8:e58194
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Rifkind, Joseph M; Salgado, Maria T; Cao, Zeling (2012) Regulation of oxygen delivery by the reaction of nitrite with RBCs under hypoxic conditions. Adv Exp Med Biol 737:183-9

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