During FY15 we accomplished the following 1) We carried out studies of mitochondrial quality and function by electron microscopy and metabolic measurements, respectively. We obtained data from 5 young and 5 older individuals. 2) We collected 10 blood cell types from 15 individuals by a combination of magnetic bead purification and flow cytometry. 3) We completed collecting RNA from peripheral blood monocytes at basal levels and after activation with LPS for various times. Activated cell RNA was assayed by microarrays and basal gene expression changes with age were assayed by RNA-Seq. Computational analyses are underway. 4) We cloned and expressed immunoglobulin heavy and light chain proteins from single plasmablasts obtained from rheumatoid arthritis (RA) patients. Several of the expressed antibodies bound PAD4, a self-antigen implicated in poor prognosis for RA treatment.
| Bektas, Arsun; Schurman, Shepherd H; Sen, Ranjan et al. (2017) Human T cell immunosenescence and inflammation in aging. J Leukoc Biol : |
| Bektas, Arsun; Sen, Ranjan; Ferrucci, Luigi (2016) Does a bit of alcohol turn off inflammation and improve health? Age Ageing : |
| Bektas, Arsun; Zhang, Yongqing; Lehmann, Elin et al. (2014) Age-associated changes in basal NF-?B function in human CD4+ T lymphocytes via dysregulation of PI3 kinase. Aging (Albany NY) 6:957-74 |
| Bektas, Arsun; Zhang, Yongqing; Wood 3rd, William H et al. (2013) Age-associated alterations in inducible gene transcription in human CD4+ T lymphocytes. Aging (Albany NY) 5:18-36 |
| Munk, Rachel B; Sugiyama, Katsuki; Ghosh, Paritosh et al. (2011) Antigen-independent IFN-? production by human naïve CD4 T cells activated by IL-12 plus IL-18. PLoS One 6:e18553 |
| Kaileh, Mary; Sen, Ranjan (2010) Role of NF-kappaB in the anti-inflammatory effects of tocotrienols. J Am Coll Nutr 29:334S-339S |
