We created a let-7 reporter assay system to investigate the function of this highly regulated microRNA in ovarian cancer. The first line of investigation targeted signaling pathways that could be elucidated by the in vitro use of a kinase inhibitor libray. Amoung the inhibitors that created a down regulation of let-7, were those that targeted glycogen synthase kinase 3 (GSK-3). To confirm this interaction, GSK3 was repressed by silencing which led in an overall increase in let-7 levels. It is possible that GSK3 might be a target for ovarian cancer therapeutics.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAG000398-06
Application #
8736561
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
2013
Total Cost
$403,501
Indirect Cost
Name
National Institute on Aging
Department
Type
DUNS #
City
State
Country
Zip Code
Lal-Nag, Madhu; Morin, Patrice J (2009) The claudins. Genome Biol 10:235
Choi, Jung-Hye; Park, Joon T; Davidson, Ben et al. (2008) Jagged-1 and Notch3 juxtacrine loop regulates ovarian tumor growth and adhesion. Cancer Res 68:5716-23