We are establishing defined molecular, biochemical, and non-invasive magnetic resonance (MR) imaging benchmarks for articular cartilage from animals and humans.
Our aim i s to demonstrate that engineered cartilage will support viability and matrix production from mammalian chondrocytes using a young bovine source, establish tissue growth which will be qualitatively similar to that produced from avian cells under comparable conditions, and establish maintenance of the hyaline phenotype. We wish to apply mechanical stimulation of chondrocytes and cartilage by application of pulsed low-intensity ultrasound (PLIUS) so as to increase expression of matrix-related mRNA and corresponding matrix production, the goal being to upregulate repair processes in an animal model of osteoarthritis (OA). Ultimately we wish to establish whether these in vitro and animal model effects will translate into treatment of OA in aging humans;that is, investigate the potential of PLIUS as a disease-modifying intervention in human subjects with early OA. Additional work involves administration of exogenous growth factors to developing tissue, both by itself and in combination with PLIUS.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAG000923-06
Application #
8736645
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
2013
Total Cost
$595,141
Indirect Cost
Name
National Institute on Aging
Department
Type
DUNS #
City
State
Country
Zip Code
Irrechukwu, Onyi N; Reiter, David A; Lin, Ping-Chang et al. (2012) Characterization of engineered cartilage constructs using multiexponential Tâ‚‚ relaxation analysis and support vector regression. Tissue Eng Part C Methods 18:433-43
Reiter, David A; Irrechukwu, Onyi; Lin, Ping-Chang et al. (2012) Improved MR-based characterization of engineered cartilage using multiexponential T2 relaxation and multivariate analysis. NMR Biomed 25:476-88
Spencer, Richard (2012) Magnetic resonance in tissue engineering. NMR Biomed 25:401
Irrechukwu, Onyi N; Lin, Ping-Chang; Fritton, Kate et al. (2011) Magnetic resonance studies of macromolecular content in engineered cartilage treated with pulsed low-intensity ultrasound. Tissue Eng Part A 17:407-15
Gurkan, I; Ranganathan, A; Yang, X et al. (2010) Modification of osteoarthritis in the guinea pig with pulsed low-intensity ultrasound treatment. Osteoarthritis Cartilage 18:724-33