Accomplishments during the year: 1. The immune response to AMA1, RON2 and EBA-175 has been studied. This work continues to expand our understanding of parasite invasion of red cells by identifying the receptors and ligands and determining the potential of these targets for vaccine development. 2. The knockout of a PfSET gene (named PfSETVS) leads to expression of all var genes, indicating that PfSETvs silences var genes. 3. Plasmodium development in the mosquito. 4. We have identified some small molecules that block junction formation and invasion.

Project Start
Project End
Budget Start
Budget End
Support Year
32
Fiscal Year
2013
Total Cost
$1,012,433
Indirect Cost
City
State
Country
Zip Code
Srinivasan, Prakash; Ekanem, Emmanuel; Diouf, Ababacar et al. (2014) Immunization with a functional protein complex required for erythrocyte invasion protects against lethal malaria. Proc Natl Acad Sci U S A 111:10311-6
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Zhu, Daming; Qian, Feng; Wu, Yimin et al. (2013) Determination of protein concentration for protein-protein conjugates using ultraviolet absorption. J Immunol Methods 387:317-21
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Tonkin, Michelle L; Arredondo, Silvia A; Loveless, Bianca C et al. (2013) Structural and biochemical characterization of Plasmodium falciparum 12 (Pf12) reveals a unique interdomain organization and the potential for an antiparallel arrangement with Pf41. J Biol Chem 288:12805-17

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