We have now shown that co-culture of Interferon alpha and gamma primed monocytes are capable of killing ovarian tumor cells both in vitro and in vivo, and the presence of both interferons is necessary for the tumorcidal effect. Further, we have shown that monocytes primed with alpha and gamma interferon combined with the standard chemotherapeutic agents Taxol and Carboplatin results in increased killing of target tumor cells. We have shown that 10 ovarian cancer cell lines are sensitive to this treatment regimen. Furthermore, we have extended this observation to show that monocytes isolated from the whole blood of healthy donors are capable of killing cells in vitro. We have also performed an advanced microarray analysis of monocytes treated with the interferons to identify which protein(s) are mediating the in vivo cell death. The array has identified a number of target genes for validation and further study

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Green, Daniel S; Nunes, Ana T; Annunziata, Christina M et al. (2016) Monocyte and interferon based therapy for the treatment of ovarian cancer. Cytokine Growth Factor Rev 29:109-15
Johnson, Chase L; Green, Daniel S; Zoon, Kathryn C (2014) Human Monocytes in the Presence of Interferons Alpha2a and Gamma Are Potent Killers of Serous Ovarian Cancer Cell Lines in Combination with Paclitaxel and Carboplatin. J Interferon Cytokine Res :
Bekisz, Joseph; Sato, Yuki; Johnson, Chase et al. (2013) Immunomodulatory effects of interferons in malignancies. J Interferon Cytokine Res 33:154-61
Miyake, Kotaro; Bekisz, Joseph; Zhao, Tongmao et al. (2012) Apoptosis-inducing factor (AIF) is targeted in IFN-α2a-induced Bid-mediated apoptosis through Bak activation in ovarian cancer cells. Biochim Biophys Acta 1823:1378-88
Baron, Samuel; Finbloom, Joel; Horowitz, Julie et al. (2011) Near eradication of clinically relevant concentrations of human tumor cells by interferon-activated monocytes in vitro. J Interferon Cytokine Res 31:569-73