There are two major types of infectious vaccinia virus particles: mature virions (MVs) and enveloped virions (EVs). The MVs, which consist of a DNA-protein core surrounded by a lipoprotein membrane, are assembled in cytoplasmic viral factories and contain about 80 polypeptides. A subpopulation of MVs is wrapped by modified trans-Golgi or endosomal cisterna containing additional viral membrane proteins, transported along microtubules to the cell periphery, and exits the cell through the plasma membrane. The EVs are essentially MVs with an additional membrane that is disrupted prior to fusion of the MV with the cell during entry.

Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
2012
Total Cost
$608,545
Indirect Cost
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State
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Moss, Bernard (2017) Investigating Viruses during the Transformation of Molecular Biology. J Biol Chem 292:3958-3969
Hyun, Seong-In; Weisberg, Andrea; Moss, Bernard (2017) Deletion of the Vaccinia Virus I2 Protein Interrupts Virion Morphogenesis, Leading to Retention of the Scaffold Protein and Mislocalization of Membrane-Associated Entry Proteins. J Virol 91:
Erlandson, Karl J; Bisht, Himani; Weisberg, Andrea S et al. (2016) Poxviruses Encode a Reticulon-Like Protein that Promotes Membrane Curvature. Cell Rep 14:2084-2091
Moss, Bernard (2016) Membrane fusion during poxvirus entry. Semin Cell Dev Biol 60:89-96
Sivan, Gilad; Weisberg, Andrea S; Americo, Jeffrey L et al. (2016) Retrograde Transport from Early Endosomes to the trans-Golgi Network Enables Membrane Wrapping and Egress of Vaccinia Virus Virions. J Virol 90:8891-905
Hyun, Seong-In; Maruri-Avidal, Liliana; Moss, Bernard (2015) Topology of Endoplasmic Reticulum-Associated Cellular and Viral Proteins Determined with Split-GFP. Traffic 16:787-95
Gjessing, Mona C; Yutin, Natalya; Tengs, Torstein et al. (2015) Salmon Gill Poxvirus, the Deepest Representative of the Chordopoxvirinae. J Virol 89:9348-67
Moss, Bernard (2015) Poxvirus membrane biogenesis. Virology 479-480:619-26
Erlandson, Karl J; Cotter, Catherine A; Charity, James C et al. (2014) Duplication of the A17L locus of vaccinia virus provides an alternate route to rifampin resistance. J Virol 88:11576-85
Maruri-Avidal, Liliana; Weisberg, Andrea S; Moss, Bernard (2013) Association of the vaccinia virus a11 protein with the endoplasmic reticulum and crescent precursors of immature virions. J Virol 87:10195-206

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