Improved vaccines against influenza would enhance our ability to decrease the deleterious effects of epidemics. This study aims to develop genetic vaccination strategies against influenza. DNA and adenoviral vaccines have been developed against different strains of influenza, including recently identified pandemic H1N1, H5N1 avian flu and seasonal flu. In addition, studies are ongoing to identify and optimize conserved regions of influenza in order to develop a vaccine that provides more universal protection. Testing has been conducted to evaluate candidates, as well as adjuvants to boost immune response, and determine the optimal strategies for immunization. Several candidates showed promising results and testing is continuing, using DNA and DNA prime with inactivated vaccine boost or nanoparticles displaying HA antigens.

Project Start
Project End
Budget Start
Budget End
Support Year
12
Fiscal Year
2013
Total Cost
$1,695,001
Indirect Cost
City
State
Country
Zip Code
Yassine, Hadi M; Boyington, Jeffrey C; McTamney, Patrick M et al. (2015) Hemagglutinin-stem nanoparticles generate heterosubtypic influenza protection. Nat Med :
Wheatley, Adam K; Whittle, James R R; Lingwood, Daniel et al. (2015) H5N1 Vaccine-Elicited Memory B Cells Are Genetically Constrained by the IGHV Locus in the Recognition of a Neutralizing Epitope in the Hemagglutinin Stem. J Immunol 195:602-10
Whittle, James R R; Wheatley, Adam K; Wu, Lan et al. (2014) Flow cytometry reveals that H5N1 vaccination elicits cross-reactive stem-directed antibodies from multiple Ig heavy-chain lineages. J Virol 88:4047-57
Kanekiyo, Masaru; Wei, Chih-Jen; Yassine, Hadi M et al. (2013) Self-assembling influenza nanoparticle vaccines elicit broadly neutralizing H1N1 antibodies. Nature 499:102-6
Ault, Alida; Zajac, Alyse M; Kong, Wing-Pui et al. (2012) Immunogenicity and clinical protection against equine influenza by DNA vaccination of ponies. Vaccine 30:3965-74
Wei, Chih-Jen; Yassine, Hadi M; McTamney, Patrick M et al. (2012) Elicitation of broadly neutralizing influenza antibodies in animals with previous influenza exposure. Sci Transl Med 4:147ra114
Wei, Chih-Jen; Boyington, Jeffrey C; McTamney, Patrick M et al. (2010) Induction of broadly neutralizing H1N1 influenza antibodies by vaccination. Science 329:1060-4
Nabel, Gary J; Fauci, Anthony S (2010) Induction of unnatural immunity: prospects for a broadly protective universal influenza vaccine. Nat Med 16:1389-91
Wei, Chih-Jen; Boyington, Jeffrey C; Dai, Kaifan et al. (2010) Cross-neutralization of 1918 and 2009 influenza viruses: role of glycans in viral evolution and vaccine design. Sci Transl Med 2:24ra21
Rao, Srinivas S; Kong, Wing-Pui; Wei, Chih-Jen et al. (2010) Comparative efficacy of hemagglutinin, nucleoprotein, and matrix 2 protein gene-based vaccination against H5N1 influenza in mouse and ferret. PLoS One 5:e9812

Showing the most recent 10 out of 11 publications