Individual gp160 clones were screened for infectivity as Env-pseudotyped viruses in a luciferase reporter gene assay in JC53-BL (TZM-bl) cells. Functional env clones were sequenced and their neutralization phenotypes characterized by using soluble CD4, monoclonal antibodies and serum samples from infected individuals and non-infected recipients of a recombinant gp120 vaccine. Env clones from geographically diverse HIV-1 isolates were selected that were not unusually sensitive or resistant to neutralization and comprised a wide spectrum of genetic, antigenic and geographic diversity. These reference reagents will facilitate proficiency testing and other validation efforts aimed at improving assay performance across laboratories and can be used for standardized assessments of vaccine elicited neutralizing antibodies. Using the reference virus regents described above, the BSL3 core virology lab has screened hundreds of animal sera generated by investigators at the VRC.

Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
2012
Total Cost
$220,536
Indirect Cost
City
State
Country
Zip Code
Bonsignori, Mattia; Kreider, Edward F; Fera, Daniela et al. (2017) Staged induction of HIV-1 glycan-dependent broadly neutralizing antibodies. Sci Transl Med 9:
Hraber, Peter; Rademeyer, Cecilia; Williamson, Carolyn et al. (2017) Panels of HIV-1 Subtype C Env Reference Strains for Standardized Neutralization Assessments. J Virol 91:
Williams, LaTonya D; Ofek, Gilad; Schätzle, Sebastian et al. (2017) Potent and broad HIV-neutralizing antibodies in memory B cells and plasma. Sci Immunol 2:
Wagh, Kshitij; Bhattacharya, Tanmoy; Williamson, Carolyn et al. (2016) Optimal Combinations of Broadly Neutralizing Antibodies for Prevention and Treatment of HIV-1 Clade C Infection. PLoS Pathog 12:e1005520
deCamp, Allan; Hraber, Peter; Bailer, Robert T et al. (2014) Global panel of HIV-1 Env reference strains for standardized assessments of vaccine-elicited neutralizing antibodies. J Virol 88:2489-507
Doria-Rose, Nicole A; Schramm, Chaim A; Gorman, Jason et al. (2014) Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies. Nature 509:55-62
Hraber, Peter; Seaman, Michael S; Bailer, Robert T et al. (2014) Prevalence of broadly neutralizing antibody responses during chronic HIV-1 infection. AIDS 28:163-9
Georgiev, Ivelin S; Doria-Rose, Nicole A; Zhou, Tongqing et al. (2013) Delineating antibody recognition in polyclonal sera from patterns of HIV-1 isolate neutralization. Science 340:751-6
Smalls-Mantey, Adjoa; Doria-Rose, Nicole; Klein, Rachel et al. (2012) Antibody-dependent cellular cytotoxicity against primary HIV-infected CD4+ T cells is directly associated with the magnitude of surface IgG binding. J Virol 86:8672-80
Doria-Rose, Nicole A; Georgiev, Ivelin; O'Dell, Sijy et al. (2012) A short segment of the HIV-1 gp120 V1/V2 region is a major determinant of resistance to V1/V2 neutralizing antibodies. J Virol 86:8319-23

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