RSV presents a significant cause of morbidity in the very young and elderly and there is currently no licensed vaccine to prevent RSV infection. The induction of both protective, disease-sparing CD8+ T cell responses and protective neutralizing antibodies are desirable in a vaccine candidate. These studies are designed to identify interventions that will increase the efficiency of nucleic acid and vector-based immunization and to optimize the conditions of each intervention. The magnitude and the quality of the immune responses induced by each vector will be compared using murine prime-challenge models. We have characterized numerous gene-based delivery vectors that express the wild-type RSV F protein. In another project Antigenicity and Immunogenicity of Stablized prefusion F protein, we have stabilized the RSV F protein in the prefusion conformation (Pre-F) and characterized the antigenicity and immunogenicity of the Pre-F protein. Currently, we are comparing vaccine regimens using DNA plasmids and gorilla adenovirus vectors expressing either the stabilized Pre-F protein or the wildtype F protein to vaccination with soluble Pre-F protein.

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14
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2016
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Liang, Bo; Ngwuta, Joan O; Surman, Sonja et al. (2017) Improved Prefusion Stability, Optimized Codon Usage, and Augmented Virion Packaging Enhance the Immunogenicity of Respiratory Syncytial Virus Fusion Protein in a Vectored-Vaccine Candidate. J Virol 91:
Liang, Bo; Ngwuta, Joan O; Herbert, Richard et al. (2016) Packaging and Prefusion Stabilization Separately and Additively Increase the Quantity and Quality of Respiratory Syncytial Virus (RSV)-Neutralizing Antibodies Induced by an RSV Fusion Protein Expressed by a Parainfluenza Virus Vector. J Virol 90:10022-10038
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Graham, Barney S; Anderson, Larry J (2013) Challenges and opportunities for respiratory syncytial virus vaccines. Curr Top Microbiol Immunol 372:391-404

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