Use of 2 lambda system to identify drug target: The Developmental Genetics Section have continued our efforts to identify targets of HIV infection using our previously developed phage based 2lambda two hybrid systems. We have cloned 5 major coat proteins of HIV virion and identified 3 pairs of interacting partners. We are in the process of setting up a high through-put screening procedure to identify small non-molecules which prevent such interactions in the virion and thus HIV assembly.
|Adhya, Sankar; Merril, Carl R; Biswas, Biswajit (2014) Therapeutic and prophylactic applications of bacteriophage components in modern medicine. Cold Spring Harb Perspect Med 4:a012518|
|Qian, Zhong; Dimitriadis, Emilios K; Edgar, Rotem et al. (2012) Galactose repressor mediated intersegmental chromosomal connections in Escherichia coli. Proc Natl Acad Sci U S A 109:11336-41|