We have produced mice that do not make mesothelin and are comparing them with normal mice to determine if various types of cancer will grow in the peritoneal cavity of these mice, where mesothelin is present on the mesothelial cells lining the peritoneal cavity of the normal mice. We have crossed these mice with immuno-deficient mice to make meso-/-, nu/nu mice to allow human cancer cells to grow in the peritoneal cavity of the mice. We have implanted mesothelin negative SHIN 3 cells into the peritoneal cavity of these mice and find they grow more slowly than in mesothelin expressing mice. We have also found that injecting these mice with MPF enhances the growth rate of these tumors. We are studying the mechanism of this effect.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC010298-16
Application #
8763065
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
16
Fiscal Year
2013
Total Cost
$65,991
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Illei, Peter B; Alewine, Christine; Zahurak, Marianna et al. (2016) Mesothelin Expression in Advanced Gastroesophageal Cancer Represents a Novel Target for Immunotherapy. Appl Immunohistochem Mol Morphol 24:246-52
Awuah, Prince; Bera, Tapan K; Folivi, Messan et al. (2016) Reduced Shedding of Surface Mesothelin Improves Efficacy of Mesothelin-Targeting Recombinant Immunotoxins. Mol Cancer Ther 15:1648-55
Liang, Xiaoyan; Schnaper, H William; Matsusaka, Taiji et al. (2016) Anti-TGF-β Antibody, 1D11, Ameliorates Glomerular Fibrosis in Mouse Models after the Onset of Proteinuria. PLoS One 11:e0155534
Okabe, Masahiro; Miyazaki, Yoichi; Niimura, Fumio et al. (2015) Unilateral ureteral obstruction attenuates intrarenal angiotensin II generation induced by podocyte injury. Am J Physiol Renal Physiol 308:F932-7
Hollevoet, Kevin; Mason-Osann, Emily; Müller, Fabian et al. (2015) Methylation-associated partial down-regulation of mesothelin causes resistance to anti-mesothelin immunotoxins in a pancreatic cancer cell line. PLoS One 10:e0122462
Acs, Peter; Bauer, Peter O; Mayer, Balazs et al. (2015) A novel form of ciliopathy underlies hyperphagia and obesity in Ankrd26 knockout mice. Brain Struct Funct 220:1511-28
Müller, Nadja; Michen, Susanne; Tietze, Stefanie et al. (2015) Engineering NK Cells Modified With an EGFRvIII-specific Chimeric Antigen Receptor to Overexpress CXCR4 Improves Immunotherapy of CXCL12/SDF-1α-secreting Glioblastoma. J Immunother 38:197-210
Hara, Satoshi; Kobayashi, Namiko; Sakamoto, Kazuo et al. (2015) Podocyte injury-driven lipid peroxidation accelerates the infiltration of glomerular foam cells in focal segmental glomerulosclerosis. Am J Pathol 185:2118-31
Stahl, Sebastian; da Silva Mateus Seidl, Ana Rita; Ducret, Axel et al. (2015) Loss of diphthamide pre-activates NF-κB and death receptor pathways and renders MCF7 cells hypersensitive to tumor necrosis factor. Proc Natl Acad Sci U S A 112:10732-7
Shah, Nirali N; Stevenson, Maryalice Stetler; Yuan, Constance M et al. (2015) Characterization of CD22 expression in acute lymphoblastic leukemia. Pediatr Blood Cancer 62:964-9

Showing the most recent 10 out of 20 publications