Abstract

Circulating glucocorticoids have been shown to be released from the adrenal gland in a highly pulsatile, or ultradian, secretion mode. The dynamics of GR interaction with regulatory elements during these cycles was found to fluctuate in concert with the changes in extracellular hormone concentration. Transcription rates for GR target genes (determined by nascent RNA) were shown to vary markedly between ultradian and constant ligand treatment regimens, leading to the postulate that the ultradian mode of hormone secretion is required for biologically accurate regulation of gene targets by GR.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC010308-12
Application #
8157269
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
12
Fiscal Year
2010
Total Cost
$501,999
Indirect Cost

  Institution

Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Lynch, Caitlin; Sakamuru, Srilatha; Huang, Ruili et al. (2017) Identifying environmental chemicals as agonists of the androgen receptor by using a quantitative high-throughput screening platform. Toxicology 385:48-58
Presman, Diego M; Hager, Gordon L (2017) More than meets the dimer: What is the quaternary structure of the glucocorticoid receptor? Transcription 8:32-39
Stavreva, Diana A; Varticovski, Lyuba; Levkova, Ludmila et al. (2016) Novel cell-based assay for detection of thyroid receptor beta-interacting environmental contaminants. Toxicology 368-369:69-79
Grøntved, Lars; Waterfall, Joshua J; Kim, Dong Wook et al. (2015) Transcriptional activation by the thyroid hormone receptor through ligand-dependent receptor recruitment and chromatin remodelling. Nat Commun 6:7048
Voss, Ty C; Hager, Gordon L (2014) Dynamic regulation of transcriptional states by chromatin and transcription factors. Nat Rev Genet 15:69-81
Dull, Angie B; George, Anuja A; Goncharova, Ekaterina I et al. (2014) Identification of compounds by high-content screening that induce cytoplasmic to nuclear localization of a fluorescent estrogen receptor ? chimera and exhibit agonist or antagonist activity in vitro. J Biomol Screen 19:242-52
Miranda, Tina B; Voss, Ty C; Hager, Gordon L (2013) High-throughput fluorescence-based screen to identify factors involved in nuclear receptor recruitment to response elements. Methods Mol Biol 1042:3-12
Stavreva, Diana A; George, Anuja A; Klausmeyer, Paul et al. (2012) Prevalent glucocorticoid and androgen activity in US water sources. Sci Rep 2:937
Biddie, Simon C; John, Sam; Sabo, Pete J et al. (2011) Transcription factor AP1 potentiates chromatin accessibility and glucocorticoid receptor binding. Mol Cell 43:145-55
Sung, Myong-Hee; McNally, James G (2011) Live cell imaging and systems biology. Wiley Interdiscip Rev Syst Biol Med 3:167-82

Showing the most recent 10 out of 14 publications