Utilization of a very small anomalous diffraction signal provided by sulfur atoms of the cysteine and methionine residues or by phosphorus atoms of nucleic acids, intrinsically present in all proteins or RNA/DNA can be successfully used to solve crystal structures of proteins. This approach, in part pioneered by us, is currently gaining increasing popularity and becomes a routine technique in the practice of macromolecular crystallography. The diffraction data to extremely high resolution in the range of 0.55-0.7 angstrom were measured using synchrotron radiation from crystals of oligonucleotides and oligopeptides, and the refined models revealeed the unprecedented structural details of the investigated molecules including electron densities at the sigma bonds between atoms. The observed very fine features in this structures will serve to improve the stereochemical libraries used for routine refinement of protein crystal structures at lower resolution.We participated in several collaborative structural projects on various biologically important proteins, such as fluorescenty proteins used as biomarkers, human mitochindrial helicase, rhizobium fucosyltransferase and plant S-adenosyl-homocysteine hydrolase.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC010378-13
Application #
8552679
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
13
Fiscal Year
2012
Total Cost
$1,418,075
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Pyra, Anna; Brzozowska, Ewa; Pawlik, Krzysztof et al. (2017) Tail tubular protein A: a dual-function tail protein of Klebsiella pneumoniae bacteriophage KP32. Sci Rep 7:2223
Luo, Zhipu; Dauter, Zbigniew (2017) Embarras de richesses - It is not good to be too anomalous: Accurate structure of selenourea, a chiral crystal of planar molecules. PLoS One 12:e0171740
Dauter, Miroslawa; Dauter, Zbigniew (2017) Many Ways to Derivatize Macromolecules and Their Crystals for Phasing. Methods Mol Biol 1607:349-356
Brzezinski, Krzysztof; Czyrko, Justyna; Sliwiak, Joanna et al. (2017) S-adenosyl-L-homocysteine hydrolase from a hyperthermophile (Thermotoga maritima) is expressed in Escherichia coli in inactive form - Biochemical and structural studies. Int J Biol Macromol 104:584-596
Dauter, Zbigniew (2017) Collection of X-Ray Diffraction Data from Macromolecular Crystals. Methods Mol Biol 1607:165-184
Ruszkowski, Milosz; Dauter, Zbigniew (2016) Structural Studies of Medicago truncatula Histidinol Phosphate Phosphatase from Inositol Monophosphatase Superfamily Reveal Details of Penultimate Step of Histidine Biosynthesis in Plants. J Biol Chem 291:9960-73
Xu, Beibei; Luo, Zhipu; Wilson, Andrew J et al. (2016) Multifunctional Charge-Transfer Single Crystals through Supramolecular Assembly. Adv Mater 28:5322-9
Sliwiak, Joanna; Dauter, Zbigniew; Jaskolski, Mariusz (2016) Crystal Structure of Hyp-1, a Hypericum perforatum PR-10 Protein, in Complex with Melatonin. Front Plant Sci 7:668
Dauter, Zbigniew; Wlodawer, Alexander (2016) Progress in protein crystallography. Protein Pept Lett 23:201-10
Ruszkowski, Milosz; Dauter, Zbigniew (2016) On methylene-bridged cysteine and lysine residues in proteins. Protein Sci 25:1734-6

Showing the most recent 10 out of 85 publications