In this project we investigate wide array of cancers: hepatocellular carcinoma, cholangiocarcinoma, pancreatic adenocarcinoma, breast cancer, melanoma, and gastric cancers This project has two prongs: One, where we use a functional approach to identify the """"""""best"""""""" cancer stem cell usiong all previously described methodes and markers and subjecting them to a rigorous tesing methodology. Once identified we will try to target these cells via the specific marker in attempt to generate novel cancer therapy. Two, using a novel methodology we try to isolate the self renwing cells via asymmetric division in various cancers and determine whether these are the """"""""fitest"""""""" cancer stem cells

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC011005-04
Application #
8349275
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2011
Total Cost
$230,132
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Xin, Hong-Wu; Ambe, Chenwi M; Miller, Tyler C et al. (2016) Liver Label Retaining Cancer Cells Are Relatively Resistant to the Reported Anti-Cancer Stem Cell Drug Metformin. J Cancer 7:1142-51
Xin, Hong-Wu; Ambe, Chenwi M; Ray, Satyajit et al. (2013) Wnt and the cancer niche: paracrine interactions with gastrointestinal cancer cells undergoing asymmetric cell division. J Cancer 4:447-57
Ray, Satyajit; Langan, Russell C; Mullinax, John E et al. (2012) Establishment of human ultra-low passage colorectal cancer cell lines using spheroids from fresh surgical specimens suitable for in vitro and in vivo studies. J Cancer 3:196-206
Mullinax, John E; Allins, Alexander; Avital, Itzhak (2011) Laparoscopic appendectomy for Amyand's hernia: a modern approach to a historic diagnosis. J Gastrointest Surg 15:533-5
Avital, Itzhak (2011) Regional chemotherapy for pancreatic cancer. J Surg Oncol 104:453