During fiscal year of 2009, we used ChIP-chip and gene expression microarray approaches to examine the role of p53 in embryonic stem cells. We surprisingly found that p53 regulates the Wnt sginaling pathway in embryonic stem cells. The Wnt signaling pathway plays important role in development and tumorigenesis. The underlying mechanism is currently under investigation in the lab. In the future, we plan to use in vivo mouse model to test our hypothesis that p53 has dual functions in embryonic stem cells.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC011158-03
Application #
8349358
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
2011
Total Cost
$404,809
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Li, Mangmang; Gou, Hongfeng; Tripathi, Brajendra K et al. (2015) An Apela RNA-Containing Negative Feedback Loop Regulates p53-Mediated Apoptosis in Embryonic Stem Cells. Cell Stem Cell 16:669-83
Shi, Jianxin; Marconett, Crystal N; Duan, Jubao et al. (2014) Characterizing the genetic basis of methylome diversity in histologically normal human lung tissue. Nat Commun 5:3365
Zhang, Xinyue; He, Yunlong; Lee, Kyoung-Hwa et al. (2013) Rap2b, a novel p53 target, regulates p53-mediated pro-survival function. Cell Cycle 12:1279-91
Shin, Min Hwa; He, Yunlong; Huang, Jing (2013) Embryonic stem cells shed new light on the developmental roles of p53. Cell Biosci 3:42
Li, Mangmang; He, Yunlong; Dubois, Wendy et al. (2012) Distinct regulatory mechanisms and functions for p53-activated and p53-repressed DNA damage response genes in embryonic stem cells. Mol Cell 46:30-42
Li, Mangmang; He, Yunlong; Feng, Xi et al. (2012) Genome-wide studies of the transcriptional regulation by p53. Biochim Biophys Acta 1819:684-7
He, Yunlong; Korboukh, Ilia; Jin, Jian et al. (2012) Targeting protein lysine methylation and demethylation in cancers. Acta Biochim Biophys Sin (Shanghai) 44:70-9
Liu, Feng; Barsyte-Lovejoy, Dalia; Allali-Hassani, Abdellah et al. (2011) Optimization of cellular activity of G9a inhibitors 7-aminoalkoxy-quinazolines. J Med Chem 54:6139-50
Huang, Jing; Dorsey, Jean; Chuikov, Sergei et al. (2010) G9a and Glp methylate lysine 373 in the tumor suppressor p53. J Biol Chem 285:9636-41
Zhang, Xinyue; Huang, Jing (2010) Integrative genome-wide approaches in embryonic stem cell research. Integr Biol (Camb) 2:510-6

Showing the most recent 10 out of 12 publications