We have mapped unique histone variant genome wide in colorectal cancer cell lines and found that that they cluster at a subset of epigenetically distinctive regions. We have mapped these regions further using cytological methods and discovered a new centromere-like region in a fragile chromosome site encompassing the myc locus. Future goals involve mapping histone variants in tumors in order to track progression of aberrant chromatin structures as a consequence of the tumorigenic process, and discovering remodelers and processes involved in the mis-localization. We are also purifying large quantities of histone variants and examining their modification status in response to tumor progression.
| Nye, Jonathan; Melters, Daniël P; Dalal, Yamini (2018) The Art of War: harnessing the epigenome against cancer. F1000Res 7:141 |
| Nuccio, A G; Bui, M; Dalal, Y et al. (2017) Mass Spectrometry-Based Methodology for Identification of Native Histone Variant Modifications From Mammalian Tissues and Solid Tumors. Methods Enzymol 586:275-290 |
| Melters, Daniël P; Nye, Jonathan; Zhao, Haiqing et al. (2015) Chromatin Dynamics in Vivo: A Game of Musical Chairs. Genes (Basel) 6:751-76 |
| Quénet, Delphine; Dalal, Yamini (2012) The CENP-A nucleosome: a dynamic structure and role at the centromere. Chromosome Res 20:465-79 |
