In collaboration with Dr. Paul Randazzo, we are studying the structure and interactions between Arf proteins and a member of the ASAP family of Arf-GAP proteins. We have produced a variety of stable-isotope variants of these proteins and established conditions for NMR spectroscopic studies. Studies are underway to provide resonance assignments, structure confirmation based on existing known structures and to determine structures which are unknown, binding studies of cofactors, and to examine the assembly of larger multi-domain and multi-component systems. New findings are emerging on the role of the PH domain within ASAP1, which we are pursuing. We are developing methods to examine these proteins in a membrane environment. We will also combine the use of SAXS data with novel NMR and EPR methods to develop three-dimensional structural models of these complexes.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC011419-03
Application #
8763511
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
2013
Total Cost
$378,671
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Gruschus, James M; Byrd, R Andrew; Randazzo, Paul A (2014) The importance of seeing surface (effects). Structure 22:363-5