As an effort to obtain specimens that might be useful in the study of the presence on specific antigens in tumor types, anonymous tissue donations were accepted. We described a method to generate multitumor blocks without specific equipment. With these tissues, we characterized the distribution of several endothelial antigens: Claudin 5, PROX1, and VEGFR2 (KDR gene product). Also distribution of KBA62 and PNL 2 antigens in melanoma and other tumors was examined. We found VEGFR2 highly specific for endothelial tumors among mesenchymal cancers, but malignant mesotheliomas were found to be unique epithelial cancers strongly positive for VEGFR2. We also examined succinate dehydrogenase deficient gastrointestinal stromal tumors, in collaboration with NCI's pediatric GIST clinic.

National Institute of Health (NIH)
National Cancer Institute (NCI)
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National Cancer Institute Division of Basic Sciences
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Miettinen, Markku; Sarlomo-Rikala, Maarit; McCue, Peter et al. (2014) Mapping of succinate dehydrogenase losses in 2258 epithelial neoplasms. Appl Immunohistochem Mol Morphol 22:31-6
Miettinen, Markku; McCue, Peter A; Sarlomo-Rikala, Maarit et al. (2014) GATA3: a multispecific but potentially useful marker in surgical pathology: a systematic analysis of 2500 epithelial and nonepithelial tumors. Am J Surg Pathol 38:13-22
Miettinen, Markku; Lasota, Jerzy (2013) Gastrointestinal stromal tumors. Gastroenterol Clin North Am 42:399-415
Killian, J Keith; Kim, Su Young; Miettinen, Markku et al. (2013) Succinate dehydrogenase mutation underlies global epigenomic divergence in gastrointestinal stromal tumor. Cancer Discov 3:648-57
Miettinen, Markku; Killian, Jonathan Keith; Wang, Zeng-Feng et al. (2013) Immunohistochemical loss of succinate dehydrogenase subunit A (SDHA) in gastrointestinal stromal tumors (GISTs) signals SDHA germline mutation. Am J Surg Pathol 37:234-40
Lasota, Jerzy; Wang, Zengfeng; Kim, Su Young et al. (2013) Expression of the receptor for type i insulin-like growth factor (IGF1R) in gastrointestinal stromal tumors: an immunohistochemical study of 1078 cases with diagnostic and therapeutic implications. Am J Surg Pathol 37:114-9