NAi has become a powerful genetic tool for dissecting genetic dependencies in cancer cells. We have developed methods and technologies to conduct rapid and cost-effective shRNA library screens to identify genetic dependencies in cancer cells.PURPOSEIn this project we will collaborate with other investigators (primarily in the NCI Intramural Program) to enable them to conduct shRNA screens in their cancer cell lines.SIGNIFICANT MATERIALS AND METHODSPooled shRNA library targeting most annotated human genes.FY2012 ACCOMPLISHMENTWe have initiated a number of shRNA screens in difference cancer cell lines and one such screen has been published already.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC011438-01
Application #
8553186
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2012
Total Cost
$134,747
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Jossé, Rozenn; Zhang, Yong-Wei; Giroux, Valentin et al. (2015) Activation of RAF1 (c-RAF) by the Marine Alkaloid Lasonolide A Induces Rapid Premature Chromosome Condensation. Mar Drugs 13:3625-39
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Zhao, Jie; Jitkaew, Siriporn; Cai, Zhenyu et al. (2012) Mixed lineage kinase domain-like is a key receptor interacting protein 3 downstream component of TNF-induced necrosis. Proc Natl Acad Sci U S A 109:5322-7