Abstract

In collaboration with Yun Wang (NIDA and NHRI, Taiwan) we used a genetically encoded calcium sensor to study the effects of methamphetamine on intracellular calcium in neurons. Through this work, it was established that direct application of methamphetamine to cortical neurons causes a rapid and transient change in intracellular calcium via L-type calcium channels and intracellular calcium stores accessed by the RyR. The work was published in Addiction Biology. In collaboration Yavin Shaham (NIDA) and Bryan Roth (UNC) we produce an AAV vector to express the KORD DREADD to study brain circuits involved in motivated behavior in rats. The study demonstrated the used of a 'retro-DREADD' dual-virus approach to restrict expression of KORD in ventral subiculum neurons that project to nucleus accumbens shell. The results indicated that the novel KORD is a promising tool to selectively inactivate brain areas and neural circuits in rat studies of motivated behavior. This work was published in Neuropsychopharmacology . In collaboration with William Freed (NIDA) we developed lentiviral vectors to study the effects of WNT3/WNT9B signaling on the differentiation of human PSCs into dopaminergic neurons. This work was published in Cell Reports. In collaboration with Antonello Bonci (NIDA) we developed an AAV vector for optogenetic control in only non-Cre expressing cells in a Cre driver mouse. Dr. Boncis lab used this vector to activate non-serotonergic neurons in the Raphe nucleus of mice to better understand the role of serotonergic vs non-serotonergic neurons of the DRN in reward circuitry. This work was published in Cell Reports. In collaboration with Dr. Henriksson (NIDA) we assisted a cell culture model that enabled studies of the interaction or REST and prodynorphin. The results suggest that REST represses PDYN expression in human SH-SY5Y cells and the adult human brain and may have implications for mental health and brain/mental disorders. This work was published in Biochim Biophys Acta.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIADA000526-08
Application #
9155751
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
2015
Total Cost
Indirect Cost

  Institution

Name
National Institute on Drug Abuse
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Anttila, Jenni E; Albert, Katrina; Wires, Emily S et al. (2018) Post-stroke Intranasal (+)-Naloxone Delivery Reduces Microglial Activation and Improves Behavioral Recovery from Ischemic Injury. eNeuro 5:
Zallar, L J; Tunstall, B J; Richie, C T et al. (2018) Development and initial characterization of a novel ghrelin receptor CRISPR/Cas9 knockout wistar rat model. Int J Obes (Lond) :
Li, Xuan; Carreria, Maria B; Witonsky, Kailyn R et al. (2018) Role of Dorsal Striatum Histone Deacetylase 5 in Incubation of Methamphetamine Craving. Biol Psychiatry 84:213-222
Richie, Christopher T; Whitaker, Leslie R; Whitaker, Keith W et al. (2017) Near-infrared fluorescent protein iRFP713 as a reporter protein for optogenetic vectors, a transgenic Cre-reporter rat, and other neuronal studies. J Neurosci Methods 284:1-14
Wallace, Veronica J; Cimbro, Raffaello; Rubio, F Javier et al. (2017) Neurons Internalize Functionalized Micron-Sized Silicon Dioxide Microspheres. Cell Mol Neurobiol 37:1487-1499
Koskela, Maryna; Bäck, Susanne; Võikar, Vootele et al. (2017) Update of neurotrophic factors in neurobiology of addiction and future directions. Neurobiol Dis 97:189-200
Sharpe, Melissa J; Marchant, Nathan J; Whitaker, Leslie R et al. (2017) Lateral Hypothalamic GABAergic Neurons Encode Reward Predictions that Are Relayed to the Ventral Tegmental Area to Regulate Learning. Curr Biol 27:2089-2100.e5
Gomez, Juan L; Bonaventura, Jordi; Lesniak, Wojciech et al. (2017) Chemogenetics revealed: DREADD occupancy and activation via converted clozapine. Science 357:503-507
Wires, Emily S; Trychta, Kathleen A; Bäck, Susanne et al. (2017) High fat diet disrupts endoplasmic reticulum calcium homeostasis in the rat liver. J Hepatol 67:1009-1017
Wires, Emily S; Henderson, Mark J; Yan, Xiaokang et al. (2017) Longitudinal monitoring of Gaussia and Nano luciferase activities to concurrently assess ER calcium homeostasis and ER stress in vivo. PLoS One 12:e0175481

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