In this year, we completed two major sub-projects. First, we employed glutamate-sensitive sensors coupled with high-speed amperometry to evaluate rapid changes in glutamate following exposure to natural arousing stimuli, cocaine and nicotine. Second, we employed glucose-sensitive sensors coupled with high-speed amperometry to evaluate rapid fluctuations in brain glucose levels following exposure to natural stimuli and drugs of abuse (cocaine and nicotine). We also initiated third project, in which we want to examine how glucose enters the brain after iv injections of several drugs of abuse with different mechanisms of action (cocaine, methamphetamine and MDPV). This work will be expanded during the next years to include more complicated behavioral paradigms that involve the use of addictive drugs.
|Wakabayashi, Ken T; Kiyatkin, Eugene A (2014) Critical role of peripheral drug actions in experience-dependent changes in nucleus accumbens glutamate release induced by intravenous cocaine. J Neurochem 128:672-85|
|Kiyatkin, Eugene A (2014) Critical role of peripheral sensory systems in mediating the neural effects of nicotine following its acute and repeated exposure. Rev Neurosci 25:207-21|
|Kiyatkin, Eugene A; Wakabayashi, Ken T; Lenoir, Magalie (2013) Physiological fluctuations in brain temperature as a factor affecting electrochemical evaluations of extracellular glutamate and glucose in behavioral experiments. ACS Chem Neurosci 4:652-65|
|Wakabayashi, Ken T; Kiyatkin, Eugene A (2012) Rapid changes in extracellular glutamate induced by natural arousing stimuli and intravenous cocaine in the nucleus accumbens shell and core. J Neurophysiol 108:285-99|