Abstract

Our interest has been first to study host-pathogen interactions between the classical periodontal pathogen P.gingivalis and antigen presenting cells of myeloid lineage and second to begin to characterize the nature of the T cell response driven by this pathogen. For this purpose we investigated the early transcriptional signatures and subsequent proteomic responses to the periodontal pathogen, Porphyromonas gingivalis (Pg) in donor-matched human blood monocytes, differentiated DC and macrophages exposed to Pg bacteria and its LPS. In our ongoing studies we are investigating the nature of the T helper cell response initiated by P. gingivalis in vitro and in vivo in an effort to uncover host molecules and cell populations which may be key in the pathogenesis of disease and which may ultimately be candidate targets for future therapeutic intervention.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIADE000736-01
Application #
8148655
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2010
Total Cost
$171,208
Indirect Cost

  Institution

Name
National Institute of Dental & Craniofacial Research
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Moutsopoulos, Niki M; Konkel, Joanne E (2018) Tissue-Specific Immunity at the Oral Mucosal Barrier. Trends Immunol 39:276-287
Konkel, J E; Moutsopoulos, N M (2018) Unique Tailoring of Th17 at the Gingival Oral Mucosal Barrier. J Dent Res 97:128-131
Dutzan, Nicolas; Abusleme, Loreto; Bridgeman, Hayley et al. (2017) On-going Mechanical Damage from Mastication Drives Homeostatic Th17 Cell Responses at the Oral Barrier. Immunity 46:133-147
Abusleme, L; Moutsopoulos, N M (2017) IL-17: overview and role in oral immunity and microbiome. Oral Dis 23:854-865
Abusleme, Loreto; Hong, Bo-Young; Hoare, Anilei et al. (2017) Oral Microbiome Characterization in Murine Models. Bio Protoc 7:
Dutzan, Nicolas; Konkel, Joanne E; Greenwell-Wild, Teresa et al. (2016) Characterization of the human immune cell network at the gingival barrier. Mucosal Immunol 9:1163-1172
Dutzan, Nicolas; Abusleme, Loreto; Konkel, Joanne E et al. (2016) Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network. J Vis Exp :53736
Konig, Maximilian F; Abusleme, Loreto; Reinholdt, Jesper et al. (2016) Aggregatibacter actinomycetemcomitans-induced hypercitrullination links periodontal infection to autoimmunity in rheumatoid arthritis. Sci Transl Med 8:369ra176
Hajishengallis, George; Moutsopoulos, Niki M (2014) Etiology of leukocyte adhesion deficiency-associated periodontitis revisited: not a raging infection but a raging inflammatory response. Expert Rev Clin Immunol 10:973-5
Moutsopoulos, Niki M; Konkel, Joanne; Sarmadi, Mojgan et al. (2014) Defective neutrophil recruitment in leukocyte adhesion deficiency type I disease causes local IL-17-driven inflammatory bone loss. Sci Transl Med 6:229ra40

Showing the most recent 10 out of 17 publications