The purpose of the current work is to understand the signaling function of the evolutionarily-conserved Gbeta5/R7-RGS/R7BP complex in humans. To this end we are first trying to identify and characterize the functions of the Gbeta5 complex in model systems such as cultured PC12 and SH-SY5Y cells, primary rodent brain neurons, and genetically-modified mice. The effect of Gbeta5 loss-of-function in neurons and brains of Gbeta5 knockout mice is currently being examined using developmental, behavioral, immunohistochemical, and neuroanatomical analyses.

Project Start
Project End
Budget Start
Budget End
Support Year
18
Fiscal Year
2011
Total Cost
$540,806
Indirect Cost
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State
Country
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Pandey, Mritunjay; Zhang, Jian-Hua; Mishra, Santosh K et al. (2017) A central role for R7bp in the regulation of itch sensation. Pain 158:931-944
Zhang, Jian-Hua; Adikaram, Poorni; Pandey, Mritunjay et al. (2016) Optimization of genome editing through CRISPR-Cas9 engineering. Bioengineered 7:166-74
Lodder, Elisabeth M; De Nittis, Pasquelena; Koopman, Charlotte D et al. (2016) GNB5 Mutations Cause an Autosomal-Recessive Multisystem Syndrome with Sinus Bradycardia and Cognitive Disability. Am J Hum Genet 99:786
Nini, Lylia; Zhang, Jian-Hua; Pandey, Mritunjay et al. (2012) Expression of the G?5/R7-RGS protein complex in pituitary and pancreatic islet cells. Endocrine 42:214-7
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Panicker, Leelamma M; Zhang, Jian-Hua; Posokhova, Ekaterina et al. (2010) Nuclear localization of the G protein beta 5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein. J Neurochem 113:1101-12