Histone methyltransferase G9a is responsible for H3K9 di-methylation (H3K9me2), an epigenetic mark for gene repression. However, the roles of G9a and G9a-mediated H3K9me2 in adipogenesis have not been reported. We report recently that G9a represses PPARgamma expression and adipogenesis. G9a regulates both positive and negative master regulators of adipogenesis: G9a represses PPARgamma expression dependent on its H3K9 methyltransferase activity while promotes Wnt expression independent of its enzymatic activity (EMBO J 2013). Together with our previous report that H3K4 methylation regulator PTIP is required for PPARgamma and C/EBPa expression and adipogenesis, these findings provide an initial view of epigenetic regulation of adipogenesis, and suggest that histone methylations control expression of positive and negative master regulators of adipogenesis.
|Kumar, Amit; Lualdi, Margaret; Loncarek, Jadranka et al. (2014) Loss of function of mouse Pax-Interacting Protein 1-associated glutamate rich protein 1a (Pagr1a) leads to reduced Bmp2 expression and defects in chorion and amnion development. Dev Dyn 243:937-47|
|Lee, Ji-Eun; Ge, Kai (2014) Transcriptional and epigenetic regulation of PPARýý expression during adipogenesis. Cell Biosci 4:29|
|Wang, Lifeng; Xu, Shiliyang; Lee, Ji-Eun et al. (2013) Histone H3K9 methyltransferase G9a represses PPAR? expression and adipogenesis. EMBO J 32:45-59|