With the goal to facilitate the global multi-method analysis (GMMA) of biophysical protein interactions data, we have further expanded and streamlined the software SEDPHAT with new simulation and analysis models. This platform offers a seamless combination of data types from different techniques, with a variety of interaction models, and specific statistical functions for the analysis of multi-component interactions in a multi-method approach. In order to facilitate the use of this analysis software for global analysis of isothermal titration calorimetry (ITC), we have further improved the new user interface specific to this technique, and developed a reference application and protocol for global analysis. To disseminate this tool and facilitate its application by colleagues, we have held multiple workshops at NIH in Bethesda, which have generated positive feedback and software improvements.

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10
Fiscal Year
2016
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Indirect Cost
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Biomedical Imaging & Bioengineering
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Zhao, Huaying; Boyd, Lisa F; Schuck, Peter (2017) Measuring Protein Interactions by Optical Biosensors. Curr Protoc Protein Sci 88:20.2.1-20.2.25
Brautigam, Chad A; Zhao, Huaying; Vargas, Carolyn et al. (2016) Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions. Nat Protoc 11:882-94
Chaires, Jonathan B; Hansen, Lee D; Keller, Sandro et al. (2015) Biocalorimetry. Methods 76:1-2
Zhao, Huaying; Piszczek, Grzegorz; Schuck, Peter (2015) SEDPHAT--a platform for global ITC analysis and global multi-method analysis of molecular interactions. Methods 76:137-148
Zhao, Huaying; Schuck, Peter (2015) Combining biophysical methods for the analysis of protein complex stoichiometry and affinity in SEDPHAT. Acta Crystallogr D Biol Crystallogr 71:3-14
Gustchina, Elena; Li, Mi; Ghirlando, Rodolfo et al. (2013) Complexes of neutralizing and non-neutralizing affinity matured Fabs with a mimetic of the internal trimeric coiled-coil of HIV-1 gp41. PLoS One 8:e78187
Keller, Sandro; Vargas, Carolyn; Zhao, Huaying et al. (2012) High-precision isothermal titration calorimetry with automated peak-shape analysis. Anal Chem 84:5066-73
Coussens, Nathan P; Schuck, Peter; Zhao, Huaying (2012) Strategies for assessing proton linkage to bimolecular interactions by global analysis of isothermal titration calorimetry data. J Chem Thermodyn 52:95-107
Zhao, Huaying; Schuck, Peter (2012) Global multi-method analysis of affinities and cooperativity in complex systems of macromolecular interactions. Anal Chem 84:9513-9
Gondeau, Claire; Corradin, Giampietro; Heitz, Frédéric et al. (2009) The C-terminal domain of Plasmodium falciparum merozoite surface protein 3 self-assembles into alpha-helical coiled coil tetramer. Mol Biochem Parasitol 165:153-61