Quantitative Mass Spectrometry incorporates collaborative projects in which the Mass Spectrometry Group provides quantitative information about, typically, small molecules by GCMS, LCMS and LCMSMS or a combination. An example of the types of projects this includes is the isoprostane analyses undertaken as part of the NIEHS led study of biomarkers of oxidative stress. We currently have several major collaborations underway. 1) with the Zeldin Lab quantitating arachidonic acid metabolites that are relevant to inflammatory, vasodilatory, endothelial protective and post-ischemic cardioprotective effects. We ahve also provided quantiative information relevant to research identifying the role of epoxyeicosatrienoic acids in the control of angiogenesis-dependent regeneration, cancer, and metastasis. 2) Investigating the relationship between levels of EETs and DHETs and blood pressure, and levels of prostaglandin E2 and pulmonary fibrosis 3) Quantitate Bisphenol A exposure from rodent bedding and diet to help assess the importance given to the estrogenic content of the animal's diet, bedding, caging, and water bottles when evaluating the estrogenic activity of bisphenol A. 4) Identify lipid/s carried by the dust mite antigen Blag1 in collaboration with G. Mueller (LSB) and Lars Pedersen (LSB) 5) Quantitate cholestenoic acid in serum as a potential marker ofseverity and length of recovery of chronic lung disease

Project Start
Project End
Budget Start
Budget End
Support Year
15
Fiscal Year
2012
Total Cost
$321,184
Indirect Cost
City
State
Country
Zip Code
Winuthayanon, Wipawee; Bernhardt, Miranda L; Padilla-Banks, Elizabeth et al. (2015) Oviductal estrogen receptor α signaling prevents protease-mediated embryo death. Elife 4:e10453
Schuck, Robert N; Theken, Katherine N; Edin, Matthew L et al. (2013) Cytochrome P450-derived eicosanoids and vascular dysfunction in coronary artery disease patients. Atherosclerosis 227:442-8
Sayers, Brian C; Taylor, Alexia J; Glista-Baker, Ellen E et al. (2013) Role of cyclooxygenase-2 in exacerbation of allergen-induced airway remodeling by multiwalled carbon nanotubes. Am J Respir Cell Mol Biol 49:525-35
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Thigpen, Julius E; Setchell, Kenneth D R; Kissling, Grace E et al. (2013) The estrogenic content of rodent diets, bedding, cages, and water bottles and its effect on bisphenol a studies. J Am Assoc Lab Anim Sci 52:130-41
Graves, Joan P; Edin, Matthew L; Bradbury, J Alyce et al. (2013) Characterization of four new mouse cytochrome P450 enzymes of the CYP2J subfamily. Drug Metab Dispos 41:763-73
Pattabiraman, Padmanabhan P; Lih, Fred B; Tomer, Kenneth B et al. (2012) The role of calcium-independent phospholipase A2γ in modulation of aqueous humor drainage and Ca2+ sensitization of trabecular meshwork contraction. Am J Physiol Cell Physiol 302:C979-91
Theken, Katherine N; Schuck, Robert N; Edin, Matthew L et al. (2012) Evaluation of cytochrome P450-derived eicosanoids in humans with stable atherosclerotic cardiovascular disease. Atherosclerosis 222:530-6
Panigrahy, Dipak; Edin, Matthew L; Lee, Craig R et al. (2012) Epoxyeicosanoids stimulate multiorgan metastasis and tumor dormancy escape in mice. J Clin Invest 122:178-91

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