Abstract

Existing two-phase solvent systems for high-speed countercurrent chromatography cover the separation of hydrophobic to moderately polar solvent systems, but often fails to provides a suitable partition coefficient values for highly polar compounds such as sulfonic dyes, catecholamines and twitter ions. The present paper introduces a new solvent series which can be applied for separation of these polar compounds by spiral tube countercurrent chromatography. It is composed of 1-butanol, ethanol, saturated ammonium sulfate and water at various volume ratios. The system consists of 10 steps which are arranged according to the polarity of the solvent system so that the two-phase solvent system with suitable K values for the target compound(s)can be found in few steps of search. Each solvent system gives proper volume ratio and high density difference between the two phases, and provides a satisfactory level of retention of the stationary phase in the spiral column assembly. The method is validated by partition coefficient measurement of four typical polar compounds including methyl green (basic dye), tartazine (sulfonic dye), tyrosine (twitter ion) and epinephrine (catecholamine), all of which show low partition coefficient values in the polar 1-butanol-water system.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAHL005107-04
Application #
8344803
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2011
Total Cost
$37,037
Indirect Cost

  Institution

Name
National Heart, Lung, and Blood Institute
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Zhang, Xinxin; Guo, Zengjun; Li, Jing et al. (2016) A new quantitation method of protodioscin by HPLC-ESI-MS/MS in rat plasma and its application to the pharmacokinetic study. Steroids 106:62-9
Shiono, Hiroyuki; Matsui, Takuya; Okada, Tadashi et al. (2016) Single-step enrichment of basophils from human peripheral blood by a novel method using a Percoll density gradient. J Sep Sci 39:3062-71
Zhang, Xinxin; Xue, Xuanji; Zhao, Jing et al. (2016) Quantitative determination of gracillin by HPLC-MS/MS after oral administration and its application to a pharmacokinetic study. Steroids 113:78-86
Zhang, Xinxin; Xue, Xuanji; Zhao, Jing et al. (2016) Diosgenin attenuates the brain injury induced by transient focal cerebral ischemia-reperfusion in rats. Steroids 113:103-12
Zhang, Xinxin; Xue, Xuanji; Xian, Liang et al. (2016) Potential neuroprotection of protodioscin against cerebral ischemia-reperfusion injury in rats through intervening inflammation and apoptosis. Steroids 113:52-63
Miyoshi, N; Kundu, S K; Tuziuti, T et al. (2016) Combination of Sonodynamic and Photodynamic Therapy against Cancer Would Be Effective through Using a Regulated Size of Nanoparticles. Nanosci Nanoeng 4:1-11
Zhang, Xinxin; Liang, Jinru; Zhang, Yongmin et al. (2015) Comparative studies on performance of CCC and preparative RP-HPLC in separation and purification of steroid saponins from Dioscorea zingiberensis C.H.Wright. J Steroids Horm Sci 6:
Dasarathy, Dhweeja; Ito, Yoichiro (2015) An improved design of spiral tube assembly for separation of proteins by high-speed counter-current chromatography. J Chromatogr A 1418:77-82
Liu, Yating; Tian, Ailin; Wang, Xiong et al. (2015) Fabrication of chiral amino acid ionic liquid modified magnetic multifunctional nanospheres for centrifugal chiral chromatography separation of racemates. J Chromatogr A 1400:40-6
Wang, Chun; Chao, Zhimao; Sun, Wen et al. (2014) ISOLATION OF GLYCOSIDES FROM THE BARKS OF ILEX ROTUNDA BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY. J Liq Chromatogr Relat Technol 37:2363-2376

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