In the past year, we have focused on dissemination of information regarding human urinary exosomes and their use in biomarker discovery studies. This has been done through review articles (see publication list below,) and through information provided on our NHLBI DIR website (Google search: Knepper Systems.) we have also maintained our Urinary Exosome Protein Database (http://dir.nhlbi.nih.gov/papers/lkem/exosome/), which has been a useful resource for the renal research community. Research on urinary exosomes in the nephrology world has exploded. Our original paper (Pisitkun T, Shen RF, Knepper MA. Identification and proteomic profiling of exosomes in human urine. Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13368-73. Epub 2004 Aug 23. PubMed PMID: 153262890) now has almost 500 citations on Scopus. Current work is limited to writing review articles on this topic.

Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
2013
Total Cost
$28,839
Indirect Cost
Name
National Heart, Lung, and Blood Institute
Department
Type
DUNS #
City
State
Country
Zip Code
Huebner, Alyssa R; Cheng, Lei; Somparn, Poorichaya et al. (2016) Deubiquitylation of Protein Cargo Is Not an Essential Step in Exosome Formation. Mol Cell Proteomics 15:1556-71
Knepper, Mark A; Kwon, Tae-Hwan; Nielsen, Soren (2015) Molecular physiology of water balance. N Engl J Med 372:1349-58
Knepper, Mark A; Kwon, Tae-Hwan; Nielsen, Soren (2015) Molecular Physiology of Water Balance. N Engl J Med 373:196
Pisitkun, Trairak; Dummer, Patrick; Somparn, Poorichaya et al. (2014) Integrated Design of Antibodies for Systems Biology Using Ab Designer. J Proteomics Bioinform 7:088-94
Miller, R Lance; Sandoval, Pablo C; Pisitkun, Trairak et al. (2013) Vasopressin inhibits apoptosis in renal collecting duct cells. Am J Physiol Renal Physiol 304:F177-88
Yu, Panpan; Pisitkun, Trairak; Wang, Guanghui et al. (2013) Global analysis of neuronal phosphoproteome regulation by chondroitin sulfate proteoglycans. PLoS One 8:e59285
Wilson, Justin L L; Miranda, Carlos A; Knepper, Mark A (2013) Vasopressin and the regulation of aquaporin-2. Clin Exp Nephrol 17:751-64
Knepper, Mark A; Miranda, Carlos A (2013) Urea channel inhibitors: a new functional class of aquaretics. Kidney Int 83:991-3
Radin, M Judith; Yu, Ming-Jiun; Stoedkilde, Lene et al. (2012) Aquaporin-2 regulation in health and disease. Vet Clin Pathol 41:455-70
Pisitkun, Trairak; Gandolfo, Maria T; Das, Samarjit et al. (2012) Application of systems biology principles to protein biomarker discovery: urinary exosomal proteome in renal transplantation. Proteomics Clin Appl 6:268-78

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