In the past year, we have continued to focus on dissemination of information regarding human urinary exosomes and their use in biomarker discovery studies. This has been done through information provided on our NHLBI DIR website (esbl.nhlbi.nih.gov) We have also maintained our (http://dir.nhlbi.nih.gov/papers/lkem/exosome/), which has been a useful resource for the renal research community. Research on urinary exosomes in the nephrology world has exploded. The PI has presented at an NIAID workshop on urinary exosomes. Our original paper (Pisitkun T, Shen RF, Knepper MA. Identification and proteomic profiling of exosomes in human urine. Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13368-73. Epub 2004 Aug 23. PubMed PMID: 153262890) now has over 1171 citations on Google Scholar. Current work is limited to writing review articles on this topic and providing protocols and antibodies to laboratories interested in using urinary exosomes as a vehicle for biomarker discovery in various disease processes.

Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
2018
Total Cost
Indirect Cost
Name
U.S. National Heart Lung and Blood Inst
Department
Type
DUNS #
City
State
Country
Zip Code
Chen, Lihe; Lee, Jae Wook; Chou, Chung-Lin et al. (2017) Transcriptomes of major renal collecting duct cell types in mouse identified by single-cell RNA-seq. Proc Natl Acad Sci U S A 114:E9989-E9998
Huebner, Alyssa R; Cheng, Lei; Somparn, Poorichaya et al. (2016) Deubiquitylation of Protein Cargo Is Not an Essential Step in Exosome Formation. Mol Cell Proteomics 15:1556-71
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Knepper, Mark A; Kwon, Tae-Hwan; Nielsen, Soren (2015) Molecular Physiology of Water Balance. N Engl J Med 373:196
Pisitkun, Trairak; Dummer, Patrick; Somparn, Poorichaya et al. (2014) Integrated Design of Antibodies for Systems Biology Using Ab Designer. J Proteomics Bioinform 7:088-94
Yu, Panpan; Pisitkun, Trairak; Wang, Guanghui et al. (2013) Global analysis of neuronal phosphoproteome regulation by chondroitin sulfate proteoglycans. PLoS One 8:e59285
Miller, R Lance; Sandoval, Pablo C; Pisitkun, Trairak et al. (2013) Vasopressin inhibits apoptosis in renal collecting duct cells. Am J Physiol Renal Physiol 304:F177-88
Wilson, Justin L L; Miranda, Carlos A; Knepper, Mark A (2013) Vasopressin and the regulation of aquaporin-2. Clin Exp Nephrol 17:751-64
Knepper, Mark A; Miranda, Carlos A (2013) Urea channel inhibitors: a new functional class of aquaretics. Kidney Int 83:991-3
Radin, M Judith; Yu, Ming-Jiun; Stoedkilde, Lene et al. (2012) Aquaporin-2 regulation in health and disease. Vet Clin Pathol 41:455-70

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